Molecular Cloning of a Monoclonal Anti-Tumor Antibody Specific for the Tn Antigen and Expression of an Active Single-Chain Fv Fragment

Autor: Otto Pritsch, Renaud Du Pasquier, Alberto Roseto, Eduardo Osinaga, Alvaro Babino, Pedro M. Alzari, Pablo Oppezzo
Přispěvatelé: Facultad de Medicina [Universidad de la Republica, Uruguay], Universidad de la República [Montevideo] (UCUR), Immuno-Hématologie et Immunopathologie, Institut Pasteur [Paris], Université de Technologie de Compiègne (UTC), Immunologie structurale, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Universidad de la República [Montevideo] (UDELAR), Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 1997
Předmět:
Models
Molecular
MESH: Carcinoma
MESH: Sequence Analysis
DNA
Antibodies
Neoplasm
Tn antigen
Immunoglobulin Variable Region
MESH: Immunoglobulin Variable Region
MESH: Amino Acid Sequence
MESH: Base Sequence
MESH: Gene Amplification
MESH: Antibodies
Monoclonal
Mice
0302 clinical medicine
Antibody Specificity
Tumor Cells
Cultured
MESH: Animals
Cloning
Molecular
Immunoglobulin Fragments
Peptide sequence
0303 health sciences
MESH: Antigens
Tumor-Associated
Carbohydrate
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Structural Biology [q-bio.BM]
Nucleic acid sequence
Antibodies
Monoclonal
MESH: Immunoglobulin M
030220 oncology & carcinogenesis
Monoclonal
Antibody
MESH: Models
Molecular
medicine.drug_class
Recombinant Fusion Proteins
MESH: Hybridomas
[PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]
Molecular Sequence Data
Immunology
MESH: Sequence Alignment
MESH: Immunoglobulin kappa-Chains
Breast Neoplasms
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Biology
Molecular cloning
Monoclonal antibody
Immunoglobulin kappa-Chains
03 medical and health sciences
Antigen
MESH: Recombinant Fusion Proteins
[CHIM.CRIS]Chemical Sciences/Cristallography
Genetics
medicine
Animals
Humans
Antigens
Tumor-Associated
Carbohydrate
MESH: Cloning
Molecular
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Amino Acid Sequence
MESH: Tumor Cells
Cultured
MESH: Antibody Specificity
MESH: Mice
MESH: Immunoglobulin Fragments
030304 developmental biology
Hybridomas
MESH: Molecular Sequence Data
MESH: Humans
Base Sequence
Carcinoma
Gene Amplification
Sequence Analysis
DNA
Molecular biology
MESH: Antibodies
Neoplasm
Immunoglobulin M
biology.protein
[INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM]
Sequence Alignment
MESH: Breast Neoplasms
Zdroj: HYBRIDOMA
HYBRIDOMA, Mary Ann Liebert, 1997, 16 (4), pp.317-324. ⟨10.1089/hyb.1997.16.317⟩
HYBRIDOMA, 1997, 16 (4), pp.317-324. ⟨10.1089/hyb.1997.16.317⟩
ISSN: 0272-457X
1554-0014
1557-8348
DOI: 10.1089/hyb.1997.16.317
Popis: International audience; We report here the first amino acid sequence of an anti-Tn monoclonal antibody raised against human breast cancer cells and show that a single chain Fv fragment of this IgM retains the Tn-binding specificity as defined by functional assays with asialo-OSM and membrane extracts from MCF-7 cells. Sequence comparisons and molecular modeling of 83D4 indicate that the antibody combining site displays a cavity-like feature primarily defined by the CDR H1 and H2 loops. This pocket could accommodate a single Tn molecule, thus, suggesting a structural explanation for the predominant expression of a particular VH gene segment in a group of antibodies that recognize tumor-associated antigens arising from an aberrant O-glycosylation.
Databáze: OpenAIRE
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