Subepithelial dendritic B cells in orofacial granulomatosis
| Autor: | Pritash Patel, Michael Escudier, Stephen Challacombe, Francesca Barone, Carlo Nunes, Jo Spencer, Jeremy D. Sanderson, Edward Odell, Laurent Boursier, Jonathan Brostoff |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Immunoglobulin gene Adolescent Somatic hypermutation Biology Immunoglobulin E Young Adult Immune system Cytidine Deaminase medicine Activation-induced (cytidine) deaminase Humans Immunology and Allergy Granulomatosis Orofacial Oral mucosa B-Lymphocytes Mouth Mucosa Gastroenterology Dendritic Cells medicine.disease medicine.anatomical_structure Immunoglobulin class switching Chronic Disease Immunology biology.protein Female Orofacial granulomatosis Immunoglobulin Heavy Chains |
| Zdroj: | Inflammatory Bowel Diseases. 16:1051-1060 |
| ISSN: | 1078-0998 |
| DOI: | 10.1002/ibd.21169 |
| Popis: | Background: Orofacial granulomatosis (OFG) is a chronic, disfiguring, granulomatous inflammation of the lips and oral mucosa. The pathogenesis is unknown, but it has been linked previously to Crohn's disease (CD) and more recently to dietary sensitivity. The oral mucosa is an immunologically responsive site associated with the generation of protective mucosal and systemic immune responses to vaccination and also hyperresponsiveness to allergens in some individuals. Classically, immune responses in oral mucosa are considered to be mediated by mucosa-associated lymphoid tissues (MALT), secondary lymphoid follicles that are intimately associated with epithelia. Methods: Immunohistochemistry was used to investigate the inflammatory infiltrate in OFG and control tissue samples. Polymerase chain reaction (PCR), cloning of PCR products, and sequencing were used to characterize the local immunoglobulin gene profile in OFG. Results: We describe large, active, dendritic B cells in oral mucosa that were not associated with any organized lymphoid tissues in the local subepithelial microenvironment. They express activation induced cytidine deaminase, which is essential for immunoglobulin gene diversification by somatic hypermutation and class switch recombination. IgE is also expressed by these B cells. They do not align with any other previously described B-cell subset in secondary lymphoid tissues in terms of morphology, proliferative activity, or phenotype. Conclusions: These subepithelial dendritic B cells may contribute to the immune responsiveness of the oral mucosa, including IgE-mediated allergic responses. In patients with OFG, further understanding of the role these cells play in oral immunity may lead to novel therapeutic possibilities. (Inflamm Bowel Dis 2010) |
| Databáze: | OpenAIRE |
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