Formulation and in vivo evaluation of probiotics-encapsulated pellets with hydroxypropyl methylcellulose acetate succinate (HPMCAS)
| Autor: | Miwon Son, Ga Hyeon Lee, Hee Jun Park, Min-Koo Choi, Yong Seok Choi, Myung Joo Kang, Joon-Ho Jun |
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| Rok vydání: | 2016 |
| Předmět: |
Male
Polymers and Plastics 02 engineering and technology Methylcellulose 030226 pharmacology & pharmacy Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Lactobacillus acidophilus Intestinal mucosa Triethyl citrate Pellet Materials Chemistry medicine Animals Food science Intestinal Mucosa Bifidobacterium biology Probiotics Organic Chemistry Plasticizer 021001 nanoscience & nanotechnology biology.organism_classification Enteric coating Rats chemistry Biochemistry 0210 nano-technology Bacteria medicine.drug |
| Zdroj: | Carbohydrate Polymers. 136:692-699 |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2015.09.083 |
| Popis: | The aim of this study was to formulate probiotics-encapsulated pellets with hydroxypropyl methylcellulose acetate succinate (HPMCAS) using a dry powder coating technique to improve the storage stability, acid resistance, and intestinal adherence of viable bacteria (Lactobacillus acidophilus and Bifidobacteria animalis ssp. Lactis). Dry coated pellet (DCP) loaded with probiotics was optimized with respect to the quantity of the HPMCAS, an enteric coating polymer (108 mg), and the kinds and amounts of plasticizer (triethyl citrate, 15.7 mg; acetylated monoglyceride, 6.8 mg), by evaluating the survival rate of the bacteria during preparation process and in an acidic medium. Dry coating process allows the whole survivals of living bacteria during preparation process. The DCP formulation exhibited markedly higher acid tolerability and storage stability compared to uncoated viable bacteria. In an in vivo mucosal adherence study in rats, a profound colonization of viable bacteria in the small and large intestine was observed in rats receiving DCP system (p0.05) compared to rats receiving uncoated probiotics. Moreover, we found that the repeated DCP administration noticeably inhibited intestinal penetration of endotoxin, a potent inflammatory stimulant, from intestinal mucus. The novel DCP system may be an alternative approach for improving bacterial viability in the preparation process and in an acidic medium, and to promote mucosal colonization of probiotic bacteria in the human gut. |
| Databáze: | OpenAIRE |
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