Podocyte Number in Children and Adults
| Autor: | Victor G. Puelles, Jinhua Li, Michael D Hughson, John F. Bertram, Peter G. Kerr, Luise A. Cullen-McEwen, Wendy E. Hoy, Rebecca N. Douglas-Denton |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Kidney Glomerulus Cell Count Stereology Autopsy Glomerulus (kidney) urologic and male genital diseases Podocyte Clinical Research Interquartile range von Willebrand Factor medicine Humans WT1 Proteins Glomerular volume Microscopy Confocal Podocytes urogenital system business.industry Infant Organ Size General Medicine Middle Aged Immunohistochemistry medicine.anatomical_structure Nephrology Child Preschool business Glomerular hyperfiltration |
| Zdroj: | Journal of the American Society of Nephrology. 26:2277-2288 |
| ISSN: | 1046-6673 |
| Popis: | Increases in glomerular size occur with normal body growth and in many pathologic conditions. In this study, we determined associations between glomerular size and numbers of glomerular resident cells, with a particular focus on podocytes. Kidneys from 16 male Caucasian-Americans without overt renal disease, including 4 children (≤3 years old) to define baseline values of early life and 12 adults (≥18 years old), were collected at autopsy in Jackson, Mississippi. We used a combination of immunohistochemistry, confocal microscopy, and design-based stereology to estimate individual glomerular volume (IGV) and numbers of podocytes, nonepithelial cells (NECs; tuft cells other than podocytes), and parietal epithelial cells (PECs). Podocyte density was calculated. Data are reported as medians and interquartile ranges (IQRs). Glomeruli from children were small and contained 452 podocytes (IQR=335-502), 389 NECs (IQR=265-498), and 146 PECs (IQR=111-206). Adult glomeruli contained significantly more cells than glomeruli from children, including 558 podocytes (IQR=431-746; P |
| Databáze: | OpenAIRE |
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