Characterization of differences between whiteflies using RAPD-PCR
| Autor: | N. J. Gawel, A. C. Bartlett |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Veterinary medicine
Insecta biology Silverleaf whitefly media_common.quotation_subject Reproducibility of Results food and beverages Genes Insect Templates Genetic Insect Whitefly Biological classification biology.organism_classification Random Amplified Polymorphic DNA Technique RAPD Crop Genetic similarity Insect Science Botany Genetics Animals Nymph Molecular Biology media_common |
| Zdroj: | Insect Molecular Biology. 2:33-38 |
| ISSN: | 1365-2583 0962-1075 |
| DOI: | 10.1111/j.1365-2583.1993.tb00123.x |
| Popis: | The B biotype of the sweetpotato whitefly, Bemisia tabaci (Gennadius) has caused over $200 million in crop damage in the US during 1992. The taxonomic classification of the A and B biotypes of B. tabaci is unclear. We used RAPD-PCR to demonstrate DNA differences between the A and B biotypes of this insect. All twenty of the RAPD primers tested distinguished readily between the biotypes. DNA extracted from individual eggs and nymphs showed identical differences. RAPD-based genetic similarity statistics indicate that these two biotypes of B. tabaci were no more similar to each other than to two other whitefly species: bayberry whitefly (Parabemisia myricae) or bandedwinged whitefly (Trialeurodes abutilonea). These results indicate that RAPD-PCR may be useful in distinguishing closely related organisms, but may not be useful in determining higher classification of insects. Before the taxonomic status of B. tabaci biotypes A and B can be determined, results from other genetic, morphological and physiological examinations will have to be compared. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text