Development of ROS ‐responsive amino acid‐based poly(ester amide) nanoparticle for anticancer drug delivery
Autor: | Chih-Chang Chu, Qinghua Xu |
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Rok vydání: | 2020 |
Předmět: |
Materials science
Polyesters Xanthones 0206 medical engineering Biomedical Engineering Antineoplastic Agents 02 engineering and technology medicine.disease_cause Biomaterials HeLa chemistry.chemical_compound Drug Delivery Systems Methionine Neoplasms medicine Humans Cytotoxicity chemistry.chemical_classification Reactive oxygen species biology Metals and Alloys Nile red 021001 nanoscience & nanotechnology biology.organism_classification 020601 biomedical engineering Cell biology chemistry Delayed-Action Preparations PC-3 Cells Cancer cell Ceramics and Composites Nanoparticles Gambogic acid Reactive Oxygen Species 0210 nano-technology Intracellular Oxidative stress HeLa Cells |
Zdroj: | Journal of Biomedical Materials Research Part A. 109:524-537 |
ISSN: | 1552-4965 1549-3296 |
Popis: | Reactive oxygen species (ROS) play an important role in cellular metabolism and many oxidative stress related diseases. Oxidative stress results from toxic effects of ROS and plays a critical role in the pathogenesis of a variety of diseases like cancers and many important biological processes. It is known that the unique feature of high intracellular ROS level in cancer cells can be considered as target and utilized as a useful cancer-related stimulus to mediate intracellular drug delivery. Therefore, biomaterials responsive to excess level of ROS are of great importance in biomedical applications. In this study, a novel ROS-responsive polymer based on L-methionine poly(ester amide) (Met-PEA-PEG) was designed, synthesized, characterized and self-assembled into nano-micellar-type nanoparticles (NP). The Met-PEA-PEG NP exhibited responsiveness to an oxidative environment. The size and morphology of the nanoparticle changed rapidly in the presence of H2 O2 . The Nile Red dye was loaded into the Met-PEA-PEG NP to demonstrate a H2 O2 concentration induced time-dependent release behavior. The Met-PEA-PEG NP was sensitive to high intracellular ROS level of PC3 prostate cancer cells. Furthermore, the Met-PEA-PEG NP was investigated as a carrier of a Chinese medicine-based anticancer component, gambogic acid (GA). Compared to free GA, the GA-loaded nanocomplex (GA-NP) showed enhanced cytotoxicity toward PC3 and HeLa cells. The GA-NP also induced a higher level of apoptosis and mitochondrial depolarization in PC3 cells than free GA. The Met-PEA-PEG NP improved the therapeutic effect of GA and may serve as a potential carrier for anticancer drug delivery. |
Databáze: | OpenAIRE |
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