A stable Escherichia coli-Mycobacterium smegmatis plasmid shuttle vector containing the mycobacteriophage D29 origin
| Autor: | M. Suissa, J. Khun, Sharon Lubinsky-Mink, M. David, A. Ben-Zvi, Shimon Ulitzur |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Genetic Vectors
Kanamycin Resistance Restriction Mapping medicine.disease_cause Origin of replication Mycobacterium Microbiology Bacteriophage Transformation Genetic Plasmid Shuttle vector Escherichia coli medicine Molecular Biology Vibrio ColE1 biology Mycobacterium smegmatis biochemical phenomena metabolism and nutrition biology.organism_classification Transformation (genetics) Genes Bacterial Luminescent Measurements DNA Transposable Elements bacteria Plasmids |
| Zdroj: | Plasmid. 28:267-271 |
| ISSN: | 0147-619X |
| DOI: | 10.1016/0147-619x(92)90059-j |
| Popis: | A plasmid shuttle vector for Escherichia coli and mycobacteria was constructed from an E. coli plasmid containing the ColE1 origin, a 2.6-kb PstI fragment from bacteriophage D29 that grows in numerous mycobacterial species, and the kanamycin resistance gene either of Tn903 or of Tn5. The resultant plasmid is 7.63 kb and can be introduced via transformation into Mycobacterium smegmatis with high efficiency. In M. smegmatis the plasmid is stable and apparently present in multiple copies. Bioluminescence (luxA and luxB of Vibrio harveyi and fischeri) has been expressed in M. smegmatis from the aminoglycoside transferase promoter of Tn5. The D29 fragment should carry an origin of replication and some associated genes that act on it since various mutations destroy the ability of this fragment to replicate in M. smegmatis. The fragment was localized on the D29 genome map. |
| Databáze: | OpenAIRE |
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