| Autor: |
Kosar Esgandari, Zakieh Rostamzadeh Khameneh, Meysam Ebrahimifar, Soraya Emamgholizadeh Minaei, Mahshid Mohammadian |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Advanced pharmaceutical bulletin. 12(2) |
| ISSN: |
2228-5881 |
| Popis: |
Purpose: Apatinib has been utilized in colon cancer therapies but its efficiency and molecular mechanism are not fully understood. Chemotherapy in combination with non-toxic compounds can be a strategy to reduce the recurrence of cancer. Consequently, this study was carried out to evaluate the effects of Apatinib and Piperine on colorectal cancer (CRC) cell line and their potential anti-cancerous mechanisms in vitro. Methods: The effects of Apatinib and Piperine on HCT-116 CRC cells were detected by assessing cell viability using MTT assay. The potential cytotoxic mechanisms of Apatinib and Piperine were investigated by evaluating the apoptosis-related gene (MDM-2) expression ratio using real-time PCR assay. Moreover, the glutathione peroxidase activity (GPX) and nitric oxide (NO) levels were assessed by colorimetric assays. Results: The proliferation rate of CRC cells decreased by increasing the concentrations of Piperine and Apatinib. When HCT-116 cells were treated with different concentrations of Apatinib in combination with Piperine, the synergistic effects were observed (Combination Index |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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