Effective genetic expression of nanoantibodies by recombinant adenoviral vector in vitro
| Autor: | Capital Em Cyrillic М Shmarov, D. Yu. Logunov, Sergei Tillib, I. L. Tutykhina, B. S. Naroditskii, I Yu Gribova, A L Gintsburg, L. V. Verkhovskaya |
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| Jazyk: | ruština |
| Rok vydání: | 2011 |
| Předmět: |
recombinant adenoviral vector
nanoantibodies genetic immunization Biology Biochemistry Virology In vitro law.invention Viral vector law Gene expression biology.protein Recombinant DNA Molecular Medicine Functional activity Vector (molecular biology) Antibody Gene Molecular Biology Biotechnology Research Article |
| Zdroj: | Acta Naturae |
| ISSN: | 2075-8251 |
| Popis: | The present study is devoted to the feasibility of expressing the single-domain mini-antibody (nanoantibody) selected from the library of sequences of the variable domains of special single-stranded antibodies derived from an immunized camel, a gene of which was introduced into eukaryotic cells within a recombinant adenoviral vector. A vector bearing the gene of a single-domain nanoantibody was obtained using the AdEasy Adenoviral Vector System (Stratagene). This method of delivering the nanoantibody gene facilitates efficient expression of this gene and functional activity of the nanoantibody. The results obtained can be used to produce passive immunizing tools against pathogens or new-generation immunobiological antitoxic medication. |
| Databáze: | OpenAIRE |
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