Clinical and epidemiological aspects of feline sporotrichosis caused by Sporothrix brasiliensis and in vitro antifungal susceptibility
| Autor: | Natalia Seron Brizzotti-Mazuchi, Taiza Maschio-Lima, Maicon Henrique Caetano, Zoilo Pires de Camargo, João Paulo Zen Siqueira, Margarete Teresa Gottardo de Almeida, Letícia Monteiro Bianco, Mariela Domiciano Ribeiro Marques, T. H. Lemes, Ruan Campos Monteiro, Bianca Gottardo de Almeida, Anderson Messias Rodrigues |
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| Přispěvatelé: | Universidade Estadual Paulista (UNESP), São José Do Rio Preto, Universidade de São Paulo (USP) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Antifungal Agents 040301 veterinary sciences Itraconazole Epidemiology Biology Cat Diseases Microbiology 0403 veterinary science 03 medical and health sciences Species Specificity Amphotericin B Zoonoses medicine Animals Antifungal agents 030304 developmental biology 0303 health sciences General Veterinary Sporotrichosis Sporothrix Broth microdilution Outbreak 04 agricultural and veterinary sciences General Medicine medicine.disease biology.organism_classification Cats Terbinafine Female Ketoconazole Brazil medicine.drug |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2022-04-29T08:45:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Sporotrichosis is a subcutaneous mycosis resulting from the traumatic implantation of pathogenic Sporothrix species. In Brazil, zoonotic transmission plays an important role in the epidemiology of the disease, involving especially cats. The objective of this study was to isolate Sporothrix spp. from cats with signs of sporotrichosis, determining the causative species, clinical and epidemiological aspects, and the in vitro susceptibility profile of the isolates against antifungal drugs. From September 2017 to February 2019, 245 samples of lesions were collected from symptomatic cats in São José do Rio Preto, Brazil. Identification of the isolates was performed by morphophysiological parameters and species-specific polymerase chain reaction. The susceptibility profile of the isolates was determined for five drugs (amphotericin B, itraconazole, ketoconazole, potassium iodide and terbinafine), using the broth microdilution method. Clinical and epidemiological aspects were analyzed based on data contained on investigation forms filled by the veterinarians at moment of collection. Sporothrix spp. were isolated in 189 (77.2%) of the samples. Phenotypic and molecular analyses revealed S. brasiliensis as the only causative agent. In vitro susceptibility testing showed lower MIC values for terbinafine (MIC = 0.03—2 μg/ml), ketoconazole (MIC = 0.03—2 μg/ml), and itraconazole (MIC = 0.03—4 μg/ml). Most of the animals were male (73.5%), adults (96.3%), stray (53.5%), and uncastrated (69.8%). Our results show the expansion of the S. brasiliensis epidemic to an area nearly 840 km apart from the epicenter of the long-lasting outbreak of cat-transmitted sporotrichosis in Rio de Janeiro. Institute of Biosciences Humanities and Exact Sciences São Paulo State University (UNESP) Department of Dermatological Infectious and Parasitic Diseases School of Medicine (FAMERP) São José Do Rio Preto Laboratory of Emerging Fungal Pathogens Department of Microbiology Immunology and Parasitology Paulista School of Medicine Federal University of São Paulo Institute of Biosciences Humanities and Exact Sciences São Paulo State University (UNESP) |
| Databáze: | OpenAIRE |
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