Characterisation of secretory calcium-binding phosphoprotein-proline-glutamine-rich 1: a novel basal lamina component expressed at cell-tooth interfaces
| Autor: | Rima Wazen, Juliana Dos Santos Neves, Pierre Moffatt, Antonio Nanci |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
DNA
Complementary Histology Molecular Sequence Data Junctional epithelium Fluorescent Antibody Technique Biology Transfection Antibodies Basement Membrane Pathology and Forensic Medicine Mice Cell Line Tumor Calcium-binding protein medicine Animals Humans Histidine Amino Acid Sequence Cloning Molecular Basement membrane Base Sequence Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Calcium-Binding Proteins Enamel organ Cell Biology Amelogenesis Blotting Northern Phosphoproteins Molar Molecular biology Recombinant Proteins Rats HEK293 Cells medicine.anatomical_structure Secretory protein Phosphoprotein Basal lamina Oligopeptides Sequence Alignment Tooth |
| Zdroj: | Cell and Tissue Research. 358:843-855 |
| ISSN: | 1432-0878 0302-766X |
| Popis: | Functional genomic screening of the rat enamel organ (EO) has led to the identification of a number of secreted proteins expressed during the maturation stage of amelogenesis, including amelotin (AMTN) and odontogenic ameloblast-associated (ODAM). In this study, we characterise the gene, protein and pattern of expression of a related protein called secretory calcium-binding phosphoprotein-proline-glutamine-rich 1 (SCPPPQ1). The Scpppq1 gene resides within the secretory calcium-binding phosphoprotein (Scpp) cluster. SCPPPQ1 is a highly conserved, 75-residue, secreted protein rich in proline, leucine, glutamine and phenylalanine. In silico data mining has revealed no correlation to any known sequences. Northern blotting of various rat tissues suggests that the expression of Scpppq1 is restricted to tooth and associated tissues. Immunohistochemical analyses show that the protein is expressed during the late maturation stage of amelogenesis and in the junctional epithelium where it localises to an atypical basal lamina at the cell-tooth interface. This discrete localisation suggests that SCPPPQ1, together with AMTN and ODAM, participates in structuring the basal lamina and in mediating attachment of epithelia cells to mineralised tooth surfaces. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink