In vitro selection of RNA aptamers to a protein target by filter immobilization
| Autor: | Andrew D. Ellington, Sulay D. Jhaveri |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
DNA
Complementary Transcription Genetic Aptamer Computational biology Biology Biochemistry Polymerase Chain Reaction Affinity Reagent RNA Aptamers Transcription (biology) Protein biosynthesis Animals Molecular Biology Selection (genetic algorithm) Base Sequence Chemistry Organic Chemistry SELEX Aptamer Technique RNA Proteins DNA Aptamers Nucleotide Molecular biology Reverse transcriptase In vitro Solubility Mutagenesis Filter (video) Protein Biosynthesis Indicators and Reagents Cattle Function (biology) Systematic evolution of ligands by exponential enrichment Filtration |
| Zdroj: | Current Protocols in Nucleic Acid Chemistry |
| ISSN: | 1934-9289 |
| Popis: | This unit describes the selection of aptamers from a pool of single-stranded RNA by binding to a protein target. Aptamers generated from this selection experiment can potentially function as protein inhibitors, and may find applications as therapeutic or diagnostic reagents. A pool of dsDNA is used to generate a ssRNA pool, which is mixed with the protein target. Bound complexes are separated from unbound reagents by filtration, and the RNA:protein complexes are amplified by a combination of reverse transcription, PCR, and in vitro transcription. |
| Databáze: | OpenAIRE |
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