CagA, a major virulence factor of Helicobacter pylori, promotes the production and underglycosylation of IgA1 in DAKIKI cells
| Autor: | Xi-sheng Xie, Shao-qing Wang, Man Yang, Fu-gang Li, Jun-ming Fan |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Glycosylation
Time Factors Blotting Western Biophysics Gene Expression Virulence Biology Biochemistry Virulence factor Helicobacter Infections Microbiology Pathogenesis fluids and secretions Bacterial Proteins Downregulation and upregulation Cell Line Tumor Humans CagA Molecular Biology Cell Proliferation Antigens Bacterial B-Lymphocytes Dose-Response Relationship Drug Helicobacter pylori Reverse Transcriptase Polymerase Chain Reaction Cell growth Glomerulonephritis IGA Cell Biology Galactosyltransferases bacterial infections and mycoses biology.organism_classification Recombinant Proteins digestive system diseases Immunoglobulin A Host-Pathogen Interactions bacteria C1GALT1 Molecular Chaperones |
| Zdroj: | Biochemical and Biophysical Research Communications. 444:276-281 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2014.01.050 |
| Popis: | While Helicobacter pylori (Hp) infection is closely associated with IgA nephropathy (IgAN), the underlying molecular mechanisms remain to be elucidated. This study was to investigate the effect of cytotoxin associated gene A protein (CagA), a major virulence factor of Hp, on the production and underglycosylation of IgA1 in the B cell line DAKIKI cells. Cells were cultured and treated with recombinant CagA protein. We found that CagA stimulated cell proliferation and the production of IgA1 in a dose-dependent and time-dependent manner. Moreover, CagA promoted the underglycosylation of IgA1, which at least partly attributed to the downregulation of β1,3-galactosyltransferase (C1GALT1) and its chaperone Cosmc. In conclusion, we demonstrated that Hp infection, at least via CagA, may participate in the pathogenesis of IgAN by influencing the production and glycosylation of IgA1 in B cells. |
| Databáze: | OpenAIRE |
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