Microscale screening of antibody libraries as maytansinoid antibody-drug conjugates

Autor:	Mikias Woldegiorgis, Fleming Michael, Chen Bai, Molly A. McShea, Seth Ettenberg, Carl Uli Bialucha, Nicholas C. Yoder, Hans K. Erickson, Kalli C. Catcott, Parmita Saxena, Stuart W. Hicks, Thomas G. Gesner, Kathy Miller, Megan E Lewis
Rok vydání:	2016
Předmět:	0301 basic medicine Drug Antibody-drug conjugate Immunoconjugates Antibodies Neoplasm media_common.quotation_subject Immunology Computational biology Antibodies Monoclonal Humanized Protein chemistry Maytansinoid 03 medical and health sciences chemistry.chemical_compound Cell Line Tumor Neoplasms Humans Immunology and Allergy Maytansine media_common biology Combinatorial chemistry body regions 030104 developmental biology chemistry biology.protein Drug Screening Assays Antitumor Antibody Reports Conjugate
Zdroj:	mAbs. 8:513-523
ISSN:	1942-0870 1942-0862
Popis:	Antibody-drug conjugates (ADCs) are of great interest as targeted cancer therapeutics. Preparation of ADCs for early stage screening is constrained by purification and biochemical analysis techniques that necessitate burdensome quantities of antibody. Here we describe a method, developed for the maytansinoid class of ADCs, enabling parallel conjugation of antibodies in 96-well format. The method utilizes ∼ 100 µg of antibody per well and requires
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78a87481b5dcb458f1953f71d0e46134 https://doi.org/10.1080/19420862.2015.1134408 Zobrazit plný text záznamu