Streptococcus salivarius as an Important Factor in Dental Biofilm Homeostasis: Influence on Streptococcus mutans and Aggregatibacter actinomycetemcomitans in Mixed Biofilm
| Autor: | Gabrijela Begić, Ivana Jelovica Badovinac, Ljerka Karleuša, Kristina Kralik, Olga Cvijanovic Peloza, Davor Kuiš, Ivana Gobin |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: |
BIOMEDICINE AND HEALTHCARE. Clinical Medical Sciences
BIOMEDICINA I ZDRAVSTVO. Dentalna medicina mixed oral biofilm A. actinomycetemcomitans S. salivarius dental biofilm dentin hydroxyapatite d-PTFE membranes BIOMEDICINA I ZDRAVSTVO. Kliničke medicinske znanosti Organic Chemistry BIOMEDICINE AND HEALTHCARE. Basic Medical Sciences General Medicine Catalysis Computer Science Applications Inorganic Chemistry BIOMEDICINE AND HEALTHCARE. Dental Medicine Physical and Theoretical Chemistry BIOMEDICINA I ZDRAVSTVO. Temeljne medicinske znanosti Molecular Biology Spectroscopy |
| Zdroj: | International Journal of Molecular Sciences; Volume 24; Issue 8; Pages: 7249 International Journal of Molecular Sciences Volume 24 Issue 8 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms24087249 |
| Popis: | A disturbed balance within the dental biofilm can result in the dominance of cariogenic and periodontopathogenic species and disease development. Due to the failure of pharmacological treatment of biofilm infection, a preventive approach to promoting healthy oral microbiota is necessary. This study analyzed the influence of Streptococcus salivarius K12 on the development of a multispecies biofilm composed of Streptococcus mutans, S. oralis and Aggregatibacter actinomycetemcomitans. Four different materials were used: hydroxyapatite, dentin and two dense polytetrafluoroethylene (d-PTFE) membranes. Total bacteria, individual species and their proportions in the mixed biofilm were quantified. A qualitative analysis of the mixed biofilm was performed using scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). The results showed that in the presence of S. salivarius K 12 in the initial stage of biofilm development, the proportion of S. mutans was reduced, which resulted in the inhibition of microcolony development and the complex three-dimensional structure of the biofilm. In the mature biofilm, a significantly lower proportion of the periodontopathogenic species A. actinomycetemcomitans was found in the salivarius biofilm. Our results show that S. salivarius K 12 can inhibit the growth of pathogens in the dental biofilm and help maintain the physiological balance in the oral microbiome. |
| Databáze: | OpenAIRE |
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