Integrated Approach for Species Identification and Quality Analysis for Labisia pumila Using DNA Barcoding and HPLC
| Autor: | Lee Suan Chua, Farah Izana Abdullah, Rosnani Hasham, Auni Aqilah Ahmad Tarmizi, Faezah Mohd Salleh, Alina Wagiran, Suraiya Binte Mostafiz |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
media_common.quotation_subject
rbcL ITS2 Labisia pumila Plant Science complex mixtures 01 natural sciences DNA barcoding Article Southeast asia Qualitative analysis herbal medicinal products (HMPs) Species identification Quality (business) Ecology Evolution Behavior and Systematics media_common Ecology Traditional medicine biology 010405 organic chemistry Botany Integrated approach biology.organism_classification 0104 chemical sciences 010404 medicinal & biomolecular chemistry QK1-989 authentication HPLC |
| Zdroj: | Plants, Vol 10, Iss 717, p 717 (2021) Plants Volume 10 Issue 4 |
| ISSN: | 2223-7747 |
| Popis: | Labisia pumila is a precious herb in Southeast Asia that is traditionally used as a health supplement and has been extensively commercialized due to its claimed therapeutic properties in boosting a healthy female reproductive system. Indigenous people used these plants by boiling the leaves however, in recent years it has been marketed as powdered or capsuled products. Accordingly, accuracy in determination of the authenticity of these modern herbal products has faced great challenges. Lack of authenticity is a public health risk because incorrectly used herbal species can cause adverse effects. Hence, any measures that may aid product authentication would be beneficial. Given the widespread use of Labisia herbal products, the current study focuses on authenticity testing via an integral approach of DNA barcoding and qualitative analysis using HPLC. This study successfully generated DNA reference barcodes (ITS2 and rbcL) for L.pumila var. alata and pumila. The DNA barcode that was generated was then used to identify species of Labisia pumila in herbal medicinal products, while HPLC was utilized to determine their quality. The findings through the synergistic approach (DNA barcode and HPLC) implemented in this study indicate the importance of both methods in providing the strong evidence required for the identification of true species and to examine the authenticity of such herbal medicinal products. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|