Re-Evaluation of Chemotherapeutic Potential of Pyoktanin Blue
Autor: | Shigeru Amano, Satoshi Yokose, Hiromu Takemura, Taisei Kanamoto, K Satoh, Daisuke Asai, Hiroshi Sakagami, Toshiko Furukawa, Kunihiko Fukuchi, Yosuke Iijima, Takuro Koshikawa |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
caspase-3
medicine.medical_treatment Cell Photodynamic therapy Caspase 3 chemotherapeutic index Article 03 medical and health sciences 0302 clinical medicine pyoktanin medicine Cytotoxicity General Environmental Science Chemistry General Engineering Neurotoxicity apoptosis oral cancer medicine.disease anti-HIV subG1 accumulation stomatognathic diseases medicine.anatomical_structure anticancer activity Cell culture Apoptosis 030220 oncology & carcinogenesis Toxicity anti-HSV Cancer research General Earth and Planetary Sciences Medicine 030217 neurology & neurosurgery |
Zdroj: | Medicines Volume 8 Issue 7 Medicines, Vol 8, Iss 33, p 33 (2021) |
ISSN: | 2305-6320 |
DOI: | 10.3390/medicines8070033 |
Popis: | Background: Pyoktanin blue (PB) is used for staining tissues and cells, and it is applied in photodynamic therapy due to its potent bactericidal activity. However, clinical application of PB as an antiviral and antitumor agent has been limited due to its potent toxicity. For clinical application, the antitumor and antiviral activity as well as the neurotoxicity of PB were re-evaluated with a chemotherapeutic index. Methods: Tumor-specificity (TS) was determined by the ratio of CC50 against normal oral cells/oral squamous cell carcinoma (OSCC) neurotoxicity by that of normal oral/neuronal cells antiviral activity by that of mock-infected/virus-infected cells and potency-selectivity expression (PSE) by dividing TS by CC50 (OSCC). Results: Antitumor activity of PB (assessed by TS and PSE) was comparable with that of DXR and much higher than that of 5-FU and melphalan. PB induced caspase-3 activation and subG1 cell accumulation in an OSCC cell line (Ca9-22). PB and anticancer drugs showed comparable cytotoxicity against both neuronal cells and OSCC cell lines. PB showed no detectable anti-HIV/HSV activity, in contrast to reverse transferase inhibitors, sulfated glucans, and alkaline extract of leaves of S.P. Conclusions: PB showed first-class anticancer activity and neurotoxicity, suggesting the importance of establishing the safe treatment schedule. |
Databáze: | OpenAIRE |
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