Mangromicins A and B: structure and antitrypanosomal activity of two new cyclopentadecane compounds from Lechevalieria aerocolonigenes K10-0216
Autor: | Kazuro Shiomi, Junya Ochiai, Satoshi Ōmura, Masato Iwatsuki, Takuji Nakashima, Atsuko Matsumoto, Aki Ishiyama, Yoshiyuki Kamiya, Kazuhiko Otoguro, Kenichiro Nagai, Yōko Takahashi |
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Rok vydání: | 2013 |
Předmět: |
Magnetic Resonance Spectroscopy
Stereochemistry Silica Gel Crystallography X-Ray High-performance liquid chromatography Mass Spectrometry Inhibitory Concentration 50 chemistry.chemical_compound Column chromatography Actinomycetales Toxicity Tests Drug Discovery Humans Moiety Cytotoxicity Lung Chromatography High Pressure Liquid Tetrahydrofuran Pharmacology Chromatography Silica gel Nuclear magnetic resonance spectroscopy Fibroblasts Trypanocidal Agents In vitro chemistry Macrolides |
Zdroj: | The Journal of Antibiotics. 67:253-260 |
ISSN: | 1881-1469 0021-8820 |
DOI: | 10.1038/ja.2013.129 |
Popis: | Two new cyclopentadecane antibiotics, named mangromicins A and B, were separated out from the culture broth of Lechevalieria aerocolonigenes K10-0216 by Diaion HP-20, silica gel and ODS column chromatography, and were finally purified by HPLC. The chemical structures of the two novel compounds were elucidated by instrumental analyses, including various NMR, MS and X-ray crystallography. Mangromicins A and B consist of cyclopentadecane skeletons with a tetrahydrofuran unit and a 5,6-dihydro-4-hydroxy-2-pyrone moiety. Mangromicins A and B showed in vitro antitrypanosomal activity with IC50 values of 2.4 and 43.4 μg ml(-1), respectively. The IC50 values of both compounds were lower than those of cytotoxicity against MRC-5 human fetal lung fibroblast cells. |
Databáze: | OpenAIRE |
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