Exposure to Traffic-Related Particulate Matter 2.5 Triggers Th2-Dominant Ocular Immune Response in a Murine Model
| Autor: | Jeong-Won Seo, Hyun Soo Lee, Sehyun Han, Ki-Joon Jeon |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
Conjunctiva genetic structures Health Toxicology and Mutagenesis air pollution lcsh:Medicine Inflammation dry eye disease (DED) Eye Article 03 medical and health sciences Mice 0302 clinical medicine Immune system medicine Animals 030304 developmental biology Corneal epithelium 0303 health sciences Goblet cell particulate matter (PM) business.industry lcsh:R Public Health Environmental and Occupational Health eye diseases Mice Inbred C57BL allergic conjunctivitis Disease Models Animal medicine.anatomical_structure Toxicity Immunology 030221 ophthalmology & optometry Cytokines Tumor necrosis factor alpha Dry Eye Syndromes Particulate Matter Lymph sense organs medicine.symptom dendritic cell (DC) business |
| Zdroj: | International Journal of Environmental Research and Public Health, Vol 17, Iss 2965, p 2965 (2020) International Journal of Environmental Research and Public Health Volume 17 Issue 8 |
| ISSN: | 1661-7827 1660-4601 |
| Popis: | Ambient particulate matter (PM), a major component of air pollution, aggravates ocular discomfort and inflammation, similarly to dry eye disease (DED) or allergies. However, the mechanism(s) by which PM induces the ocular inflammatory response is unknown. This study investigated the immunological response of traffic-related fine particulate matter (PM2.5) on the ocular surface in a murine model. C57BL/6 mice were exposed by topical application to PM2.5 or vehicle for 14 days to induce experimental environmental ocular disease. Corneal fluorescein staining and the number of ocular inflammatory cells were assessed in both groups. The expression of IL-1&beta IL-6, tumor necrosis factor (TNF)-&alpha and mucin 5AC (MUC5AC) in the ocular surface were evaluated by real-time PCR. An immunohistochemical assay evaluated apoptosis and goblet cell density. ELISA was used to determine the levels of serum IgE and cytokines of Type 1 helper (Th1) and Type 2 helper (Th2) cells after in vitro stimulation of T cells in the draining lymph nodes (LNs). Exposure to traffic-related PM2.5 significantly increased corneal fluorescein staining and cellular toxicity in the corneal epithelium compared with the vehicle control. A significant increase in the number of CD11b+ cells on the central cornea and mast cells in the conjunctiva was observed in the PM2.5 group. Exposure to PM2.5 was associated with a significant increase in the corneal or conjunctival expression of IL-1&beta IL-6, TNF, and MUC5AC compared to the vehicle, and increased maturation of dendric cells (DCs) (MHC-IIhighCD11c+) in draining LNs. In addition, PM2.5 exposure increased the level of serum IgE and Th2 cytokine production in draining LNs on day 14. In conclusion, exposure to traffic-related PM2.5 caused ocular surface damage and inflammation, which induced DC maturation and the Th2-cell-dominant allergic immune response in draining LNs. |
| Databáze: | OpenAIRE |
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