Culture and establishment of self-renewing human liver 3D organoids with high uric acid for screening antihyperuricemic functional compounds
| Autor: | Wanqian Sha, Yang Hu, Jiaoyan Ren, William Kwame Amakye, Zhenzhen Xu, Maojin Yao, Chuanli Hou |
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| Rok vydání: | 2022 |
| Předmět: |
Human liver
Functional features nutritional and metabolic diseases Allopurinol Hyperuricemia General Medicine Pharmacology medicine.disease In vitro Gout Suppressants Uric Acid Analytical Chemistry Organoids chemistry.chemical_compound Liver chemistry Organoid medicine Humans Uric acid Purine metabolism Food Science medicine.drug |
| Zdroj: | Food Chemistry. 374:131634 |
| ISSN: | 0308-8146 |
| Popis: | Hyperuricemia (HUA) is a metabolic disease caused by disorders of purine metabolism, the prevalence of which has increased worldwide. At present, most drugs aimed at lowering uric acid have toxic side effects, and in vitro screening of uric acid-lowering active substances are inefficient. Here, a long-term 3D human liver organoid culture system with high uric acid for screening and evaluating the efficacy of uric acid-lowering functional compounds. This liver organoids can be established from single hepatocytes and grown for multiple months, while retaining key morphological and functional features. Furthermore, establishment of HUA organoids model was verified by antihyperuricemic drugs allopurinol, as well as reported bioactive peptides, which significantly reduced uric acid production in the liver organoids (p 0.05). The results demonstrated that it has the potential to be used as a rapid and valid in vitro model to screen antihyperuricemic compounds that mimics in vivo cell growth patterns. |
| Databáze: | OpenAIRE |
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