Red and White Wine Lees Show Inhibitory Effects on Liver Carcinogenesis
| Autor: | Zahira Fernández-Bedmar, Pilar Delgado de la Torre, Ángeles Alonso-Moraga, María Dolores Luque de Castro, Marina Sánchez-Frías, Silvia Guil-Luna, Yolanda Millán-Ruiz, Jaouad Anter |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Organoleptic Catechols Wine Pyrogallol digestive system Lees Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Liver Neoplasms Experimental Alu Elements Gallic Acid Animals Anticarcinogenic Agents Diethylnitrosamine Food science Winemaking 030109 nutrition & dietetics Body Weight food and beverages Catechin Organ Size DNA Methylation biochemical phenomena metabolism and nutrition Syringic acid humanities 030104 developmental biology Liver chemistry White Wine Polyphenol Fermentation embryonic structures Food Science Biotechnology |
| Zdroj: | Molecular Nutrition & Food Research. 63:1800864 |
| ISSN: | 1613-4133 1613-4125 |
| Popis: | Scope Wine has shown anticarcinogenic benefits in hepatocarcinoma and polyphenols seem to be responsible for these effects. Wine lees are the sediments produced during fermentation and they endow wine with organoleptic and physicochemical properties. However, the anticarcinogenic role of these compounds is still unknown. Thus, the purpose of this work is to determine the phytochemical profiles of wine lees and then to analyze their anticarcinogenic effect and DNA methylation on a model of hepatocarcinogenesis. Methods and results The phytochemical composition of lees is determined by the Folin-Ciocalteu method and high-performance liquid chromatography. An in vivo study using a diethyl nitrosamine-hepatocarcinogenesis-induced model is performed to investigate the hepatoprotective properties of different doses of wine lees. For the DNA methylation analysis, a bisulfite-based method is used. Both types of lees mostly contain pyrogallol, gallic, and syringic acid with a high content of catechins in red lees. The carcinogen hypermethylates the Alu-M2 repetitive sequence and white lees decreases the hypermethylation at all tested concentrations. Low concentration of red and white lees and high concentration of white lees significantly improve the hepatocellular architecture and decrease the mitotic index in the murine model. Conclusion These findings suggest that wine lees are promising agents for chemoprevention of hepatocarcinoma. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text