Increased interleukin-1 activity in the injured vitamin A-deficient cornea
| Autor: | Laila A. Hanninen, Naveed Shams, Kenneth R. Kenyon, Kiyoshi Watanabe, Chittaranjan V. Reddy, Salwa A. Elgebaly |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Vitamin
Male medicine.medical_specialty Neutrophils Inflammation Corneal inflammation Pathogenesis Cornea Rats Sprague-Dawley chemistry.chemical_compound Mice Internal medicine medicine Animals Corneal Ulcer Mice Inbred C3H business.industry Interleukin-6 Vitamin A Deficiency Interleukin Chemotaxis Fibroblasts medicine.disease eye diseases Rats Vitamin A deficiency Ophthalmology Chemotaxis Leukocyte medicine.anatomical_structure Endocrinology chemistry sense organs medicine.symptom business Interleukin-1 |
| Zdroj: | Cornea. 13(2) |
| ISSN: | 0277-3740 |
| Popis: | Injury to a vitamin A-deficient cornea leads to severe acute inflammation often culminating in ulceration. We report on possible regulatory mechanisms involved in the pathogenesis of corneal inflammation in vitamin A deficiency. Thymocyte comitogenic assay and interleukin (IL)-6 induction in corneal fibroblasts have shown that thermally injured and mechanically abraded vitamin A-deficient rat corneas produce much higher levels of an IL-1-like factor as compared with uninjured or injured, normal control corneas. This was confirmed by antibody capture enzyme immunoassay, which detected high levels of IL-1 alpha and IL-1 beta in injured vitamin A-deficient corneas. To our knowledge this is the first report describing the induction of IL-1 in the vitamin A-deficient cornea by thermal and mechanical injuries. When mechanically injured corneas were screened for chemotactic activity, they were found to contain significantly higher levels of a chemoattractant as compared with similarly injured, normal control corneas. Chemotactic activity [expressed as a percentage of a known chemotactic tripeptide, formyl-methionyl-leucyl-phenylalanine (fMLP), found in medium harvested from vitamin A-deficient corneas] averaged 58.8 +/- 8.9% (SEM) as compared with 12.6 +/- 5.4% in medium conditioned by normal corneas. Checkerboard analysis confirmed that the activity in vitamin A-deficient cornea conditioned medium was chemotactic and not chemokinetic. These results demonstrate a correlation between IL-1 levels and severity of inflammation in the injured vitamin A-deficient rat cornea. |
| Databáze: | OpenAIRE |
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