Genetic Diversity and Virulence of Wheat and Barley Strains of Xanthomonas translucens from the Upper Midwestern United States
Autor: | Rebecca D. Curland, Boris A. Vinatzer, Leon Van Eck, Carolee T. Bull, Carol A. Ishimaru, Ruth Dill-Macky, Liangliang Gao |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Xanthomonas 030106 microbiology Virulence Plant Science Plant disease resistance Biology Midwestern United States Microbiology 03 medical and health sciences Bacterial Proteins Genetic variation Phylogeny Triticum Bacterial leaf streak Plant Diseases Genetics Genetic diversity Genetic Variation food and beverages Bayes Theorem Hordeum Sequence Analysis DNA biology.organism_classification Xanthomonas translucens Host-Pathogen Interactions Multilocus sequence typing Agronomy and Crop Science Multilocus Sequence Typing |
Zdroj: | Phytopathology®. 108:443-453 |
ISSN: | 1943-7684 0031-949X |
Popis: | Bacterial leaf streak (BLS) of wheat and barley, caused by Xanthomonas translucens pv. undulosa and X. translucens pv. translucens, has been of growing concern in small grains production in the Upper Midwestern United States. To optimize disease resistance breeding, a greater awareness is needed of the pathovars and genetic diversity within the pathogens causing BLS in the region. Multilocus sequencing typing (MLST) and analysis (MLSA) of four common housekeeping genes (rpoD, dnaK, fyuA, and gyrB) was used to evaluate the genetic diversity of 82 strains of X. translucens isolated between 2006 and 2013 from wheat, barley, rye, and intermediate wheatgrass. In addition, in planta disease assays were conducted on 75 strains to measure relative virulence in wheat and barley. All strains were determined by MLSA to be related to X. translucens pv. undulosa and X. translucens pv. translucens. Clustering of strains based on Bayesian, network, and minimum spanning trees correlated with relative virulence levels in inoculated wheat and barley. Thus, phylogeny based on rpoD, dnaK, fyuA, and gyrB correlated with host of isolation and was an effective means for predicting virulence of strains belonging to X. translucens pv. translucens and X. translucens pv. undulosa. |
Databáze: | OpenAIRE |
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