Inhibition of Staphylococcal Wound Infection and Potentiation of Antibiotic Prophylaxis by a Recombinant Fragment of the Fibronectin‐Binding Protein ofStaphylococcus aureus
| Autor: | Barbara E. Menzies, Douglas S. Kernodle, Yordanka Kourteva, Allen B. Kaiser |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Staphylococcus aureus Umbilical Veins medicine.drug_class Guinea Pigs Antibiotics Cefazolin Biology medicine.disease_cause Microbiology Bacterial Proteins medicine Animals Humans Immunology and Allergy Antibiotic prophylaxis Adhesins Bacterial Cells Cultured Infectious dose Drug Synergism Antibiotic Prophylaxis Staphylococcal Infections Entry into host Recombinant Proteins Cephalosporins Disease Models Animal Infectious Diseases Fibronectin binding Wound Infection Female Endothelium Vascular Carrier Proteins Staphylococcus medicine.drug |
| Zdroj: | The Journal of Infectious Diseases. 185:937-943 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1086/339484 |
| Popis: | Adherence of Staphylococcus aureus to host tissues is a critical step for colonization and initiation of infection. The fibronectin-binding proteins (FnBPs) of S. aureus have been implicated in adherence and internalization in nonprofessional phagocytes. A recombinant fragment of the fibronectin-binding domains (rFnBF) that potently inhibits S. aureus entry into host cells was generated. To test the hypothesis that rFnBF may attenuate the establishment of infection, the ability of intermuscularly administered rFnBF to prevent abscess formation was determined in a guinea pig model of wound infection. rFnBF exhibited dose-dependent inhibition of abscess formation and, at a 100-microg dose, raised the median infective dose approximately 170-fold, compared with the control. In addition, rFnBF potentiated the benefit of prophylaxis with cefazolin. Thus, exogenous administration of the fibronectin-binding domain of FnBP reduces the risk of staphylococcal abscess formation and should be investigated further as a novel agent for prevention of wound infection. |
| Databáze: | OpenAIRE |
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