Possible role of IGF2 receptors in regulating selection of 2 dominant follicles in cattle selected for twin ovulations and births
| Autor: | Pauline Y. Aad, Leon J. Spicer, Sherrill E. Echternkamp |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Ovulation
endocrine system medicine.medical_specialty Granulosa cell Biology Receptor IGF Type 2 Endocrinology Ovarian Follicle Food Animals Pregnancy Internal medicine medicine Animals Humans RNA Messenger Androstenedione Selection Genetic Progesterone Estrous cycle Granulosa Cells Estradiol Insulin-like growth factor 2 receptor luteinizing hormone/choriogonadotropin receptor Receptors LH Follicular fluid Follicular Fluid Theca Theca Cells Pregnancy Twin Receptors FSH Cattle Female Animal Science and Zoology Follicle-stimulating hormone receptor |
| Zdroj: | Domestic Animal Endocrinology. 45:187-195 |
| ISSN: | 0739-7240 |
| DOI: | 10.1016/j.domaniend.2013.09.001 |
| Popis: | Abundance of IGF-2 receptor (IGF2R), FSH receptor (FSHR), and LH receptor (LHCGR) mRNA in granulosa cells (GCs) or theca cells (TCs) or both cells as well as estradiol (E2), progesterone (P4), and androstenedione concentrations in follicular fluid were compared in cows genetically selected (Twinner) or not selected (control) for multiple ovulations and twin births. Cows were slaughtered at day 3 to 4 (day 3) and day 5 to 6 (day 5) of an estrous cycle, and ovaries, follicular fluid, GCs, and TCs were collected. The two largest (F1 and F2) E2-active (EA) and E2-inactive (EI) follicles were selected according to their E2-to-P4 ratio and diameter. Androstenedione levels in EA F1 and F2 follicles were 5-fold greater (P0.05) in Twinner cows than in control cows on day 3 but did not differ on day 5. Twinner cows also had greater (P0.05) E2 and P4 concentrations, whereas steroid levels in EI follicles did not differ (P0.10) between genotypes. In EA F2 follicles, IGF2R levels in GCs were greater (P0.05) in control cows than in Twinner cows on day 3 and day 5, whereas IGF2R mRNA in TCs did not differ (P0.10). On day 3, FSHR mRNA levels were greater (P0.05) in GCs of EA F1 and EI F2 follicles of control cows than of Twinner cows. LH receptor mRNA expression was less in GCs and greater in TCs of EA F2 follicles in control cows than in Twinner cows (P0.05). We hypothesize that reduced GC IGF2R expression in F2 follicles of Twinner cows may play a role in the development of 2 or more dominant follicles. |
| Databáze: | OpenAIRE |
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