How much separation for LC-MS/MS quantitative bioanalysis of drugs and metabolites?
| Autor: | Aimin Tan, John C. Fanaras |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Bioanalysis
Curcumin Clinical Biochemistry Ion suppression in liquid chromatography–mass spectrometry Tandem mass spectrometry 030226 pharmacology & pharmacy 01 natural sciences Biochemistry Sensitivity and Specificity Analytical Chemistry 03 medical and health sciences 0302 clinical medicine Isomerism Tandem Mass Spectrometry Lc ms ms Humans Dronabinol Chromatography Chemistry 010401 analytical chemistry Reproducibility of Results Cell Biology General Medicine Capacity factor 0104 chemical sciences Models Chemical Pharmaceutical Preparations Linear Models Chromatography Liquid |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 1084 |
| ISSN: | 1873-376X |
| Popis: | LC-MS/MS has been the dominant analytical technology for quantitative bioanalysis of drugs and metabolites for more than two decades. Despite this, a very fundamental question like how much separation is required for LC-MS/MS quantitative bioanalysis of drugs and metabolites has not been adequately addressed. Some think that no or only very limited separation is necessary thanks to the unparalleled selectivity offered by tandem mass spectrometry. Others think that the more separation, the better, because of the potential detrimental impact of matrix effect (ion suppression or enhancement). Still others just use a rule-of-thumb approach by keeping the adjusted retention/capacity factor always between 2 and 5. The purpose of this article is to address this fundamental question through rational thinking together with various real case examples drawn from regulated bioanalytical laboratories. |
| Databáze: | OpenAIRE |
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