The Organellar Genome and Metabolic Potential of the Hydrogen-Producing Mitochondrion of Nyctotherus ovalis
Autor: | Carola Burgtorf, Johannes H. P. Hackstein, Theo A. van Alen, Guenola Ricard, Aloysius G.M. Tielens, Georg W.M. van der Staay, Martijn A. Huynen, Isabel Duarte, Jan W. P. Kuiper, Bas E. Dutilh, Rob M. de Graaf |
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Přispěvatelé: | Medical Microbiology & Infectious Diseases |
Rok vydání: | 2011 |
Předmět: |
0106 biological sciences
Gene Transfer Horizontal Hydrogenosome Mitochondrial disease hydrogenosome Genes Protozoan Protozoan Proteins adaptation Mitochondrion Biology 010603 evolutionary biology 01 natural sciences Genome 03 medical and health sciences Ecogenomics Complementary DNA evolution Organelle Genetics medicine mitochondrion Ciliophora Inner mitochondrial membrane Molecular Biology Gene Research Articles Phylogeny Ecology Evolution Behavior and Systematics 030304 developmental biology Nyctotherus Cell Nucleus Organelles 0303 health sciences medicine.disease Biological Evolution Mitochondria Genome Mitochondrial Energy and redox metabolism Mitochondrial medicine [NCMLS 4] horizontal gene transfer Hydrogen |
Zdroj: | Molecular Biology and Evolution Molecular Biology and Evolution, 28(8), 2379-2391. Oxford University Press Molecular Biology and Evolution, 28, 2379-2391 Molecular Biology and Evolution, 28, 8, pp. 2379-2391 |
ISSN: | 1537-1719 0737-4038 |
Popis: | It is generally accepted that hydrogenosomes (hydrogen-producing organelles) evolved from a mitochondrial ancestor. However, until recently, only indirect evidence for this hypothesis was available. Here, we present the almost complete genome of the hydrogen-producing mitochondrion of the anaerobic ciliate Nyctotherus ovalis and show that, except for the notable absence of genes encoding electron transport chain components of Complexes III, IV, and V, it has a gene content similar to the mitochondrial genomes of aerobic ciliates. Analysis of the genome of the hydrogen-producing mitochondrion, in combination with that of more than 9,000 genomic DNA and cDNA sequences, allows a preliminary reconstruction of the organellar metabolism. The sequence data indicate that N. ovalis possesses hydrogen-producing mitochondria that have a truncated, two step (Complex I and II) electron transport chain that uses fumarate as electron acceptor. In addition, components of an extensive protein network for the metabolism of amino acids, defense against oxidative stress, mitochondrial protein synthesis, mitochondrial protein import and processing, and transport of metabolites across the mitochondrial membrane were identified. Genes for MPV17 and ACN9, two hypothetical proteins linked to mitochondrial disease in humans, were also found. The inferred metabolism is remarkably similar to the organellar metabolism of the phylogenetically distant anaerobic Stramenopile Blastocystis. Notably, the Blastocystis organelle and that of the related flagellate Proteromonas lacertae also lack genes encoding components of Complexes III, IV, and V. Thus, our data show that the hydrogenosomes of N. ovalis are highly specialized hydrogen-producing mitochondria. |
Databáze: | OpenAIRE |
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