Differential expression of the Ca2+ binding S100A6 protein in normal, preneoplastic and neoplastic colon mucosa
| Autor: | B.W Schäfer, Jiří Stulík, Jan Österreicher, F Langr, P Jandı́k, C.W Heizmann, J Bureš, Jiří Knížek, K Dědič, Kamila Koupilova |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adenoma
Gene isoform Cancer Research Pathology medicine.medical_specialty Colorectal cancer Blotting Western Cell Cycle Proteins Biology S100 Calcium Binding Protein A6 Tumor Cells Cultured medicine Pi Humans Protein Isoforms Electrophoresis Gel Two-Dimensional Intestinal Mucosa Calcium-Binding Proteins S100 Proteins Cancer medicine.disease Immunohistochemistry Staining Blot Oncology Colonic Neoplasms Ca2 binding Colorectal Neoplasms Precancerous Conditions |
| Zdroj: | European Journal of Cancer. 36:1050-1059 |
| ISSN: | 0959-8049 |
| DOI: | 10.1016/s0959-8049(00)00043-5 |
| Popis: | The expression of calcium-binding protein S100A6 was investigated in normal colon tissue, in colon adenomas and in colorectal carcinomas. Using an immunoblotting approach we detected four S100A6 variants with Mwt of 10 kDa and pI of 5.05 (isoform I), 5.15 (isoform II), 5.23 (isoform III) and 5.32 (isoform IV) that were differentially expressed in the analysed samples. The quantitative examination of S100A6 variant expression in 25 pairs of colorectal carcinoma and matched control mucosa proved a statistically significant increased abundance of S100A6 isoforms I (P = 0.004) and III (P = 0.025) in malignant tissue, and conversely, an increased level of S100A6 isoform IV in healthy tissue (P = 0.022). The expression of isoforms I and III and the loss of isoform IV were also observed in colon cancer cell lines. In addition, the immunohistochemical study of 16 primary colorectal carcinomas revealed both in the non-paired Student t-test and in the Mann Whitney test the statistically significant accumulation of S100A6 protein (P < 0.001) in the invasive margin of the tumour. The immunohistochemical analysis of S100A6 protein in polyps differing in clinical severity gave a strong staining that was maximal in dysplastic lesions. Thus, our results indicate a possible, statistically significant correlation (non-paired Student t-test P = 0.036) between S100A6 expression and colon carcinoma progression. |
| Databáze: | OpenAIRE |
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