Prognostic significance of RBP2-H1 variant of JARID1B in melanoma

Autor: Barbara W. Chwirot, Łukasz Kuźbicki, Dariusz Lange, Agata Stanek-Widera
Rok vydání: 2017
Předmět:
0301 basic medicine
Jumonji Domain-Containing Histone Demethylases
Cancer Research
Skin Neoplasms
Cellular differentiation
Context (language use)
Kaplan-Meier Estimate
Patient’s survival
lcsh:RC254-282
Epigenesis
Genetic
Malignant transformation
03 medical and health sciences
0302 clinical medicine
Biomarkers
Tumor
Genetics
medicine
Humans
Protein Isoforms
Epigenetics
Melanoma
biology
Nuclear Proteins
Prognosis
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
medicine.disease
Immunohistochemistry
Gene Expression Regulation
Neoplastic
Repressor Proteins
Alternative Splicing
JARID1B
030104 developmental biology
Oncology
Lymphatic Metastasis
030220 oncology & carcinogenesis
Cutaneous melanoma
Disease Progression
Cancer research
biology.protein
Demethylase
RBP2-H1 variant of JARID1B
Research Article
Zdroj: BMC Cancer, Vol 17, Iss 1, Pp 1-7 (2017)
BMC Cancer
ISSN: 1471-2407
DOI: 10.1186/s12885-017-3836-x
Popis: Background Histone demethylase JARID1B plays several context dependent roles in epigenetic regulation of cellular differentiation in normal development and is highly expressed in multiple human cancers. The protein is a strong transcriptional repressor capable of downregulating numerous genes. There are three splicing isoforms of JARID1B, however the links between the protein structure and function are not clear. The expression pattern of JARID1B in human melanoma seems to be different from observed in other cancers. Moreover, up to now no data on the impact of JARID1B expression in cutaneous melanoma on the patients’ prognosis have been reported. Methods We investigated immunohistochemically the association of intratumoral expression of total JARID1B protein and its RBP2-H1 isoform in primary and metastatic melanomas with prognosis for the patients. Results Expression of both total JARID1B protein and its RBP2-H1 variant was found in all the melanomas investigated. Our results indicate, however, that only high (above 90% of the cells) intratumoral expression of RBP2-H1 can be considered prognostic factor associated with worse overall survival of the patients. Conclusions Such results if considered together with data demonstrating a switch to enhanced expression of RBP2-H1 at early stages of malignant transformation of melanocytes are in agreement with hypothetical crucial role of JARID1B in the course of melanoma development and progression and suggest that altered splicing of JARID1B may be important factor increasing melanoma aggressiveness. Electronic supplementary material The online version of this article (10.1186/s12885-017-3836-x) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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