Correlation between elevated inflammatory cytokines of spleen and spleen index in acute spinal cord injury

Autor: Xiao-Yan Ding, Xiao-Hui Li, Min-Jie Gong, Jia-Qi An, Feng Wu, Sheng-Li Huang
Rok vydání: 2020
Předmět:
Zdroj: Journal of Neuroimmunology. 344:577264
ISSN: 0165-5728
DOI: 10.1016/j.jneuroim.2020.577264
Popis: Background Spinal cord injury (SCI) is a devastating disorder. After SCI, it initiates a robust immune response. Considering the spleen is one of the most important immune organs, the present study further characterizes the inflammatory cytokine profile of spleen in acute SCI. Methods Adult rats were divided into sham and SCI groups (n = 36). SCI was produced at the T3 vertebral level. The whole blood and spleen was collected at 6, 24, 48, 72, 120, and 168 h after SCI. The levels of the inflammatory factors (IL-1β, IL-6, IL-10, TNF-α, and TGF-β) in spleen and serum were measured with an ELISA kit. Results The results showed significantly elevated levels of IL-1β, IL-6, IL-10 and TNF-α in spleen compared with control group levels. Inflammatory cytokine levels of spleen correlated negatively with spleen index. Conclusion It was found that inflammatory cytokines in spleen showed dynamic responses to SCI, which suggest their specificity change of spleen caused by SCI. These results suggest that a possible involvement of spleen in the initiation of the inflammatory response after SCI.
Databáze: OpenAIRE
Externí odkaz: