UPLC-MS metabolome based classification of Lupinus and Lens seeds: A prospect for phyto-equivalency of its different accessions
| Autor: | Andreas G. Heiss, Matthias Kropf, Amira R. Khattab, Amal A. Maamoun, Mohamed A. Farag |
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| Rok vydání: | 2019 |
| Předmět: |
030309 nutrition & dietetics
Biology Health benefits 03 medical and health sciences Lupinus Alkaloids 0404 agricultural biotechnology Metabolomics Phenols Functional Food Tandem Mass Spectrometry Botany Lupinus hispanicus Metabolome Cultivar Flavonoids Sphingolipids 0303 health sciences Plant Extracts Fatty Acids food and beverages 04 agricultural and veterinary sciences Fabaceae Saponins biology.organism_classification 040401 food science Austria Multivariate Analysis Seeds Lens Plant Uplc ms ms Chromatography Liquid Food Science |
| Zdroj: | Food Research International. 115:379-392 |
| ISSN: | 0963-9969 |
| Popis: | Fabaceae is well-known for its seed nutritious and bioactive composition as exemplified by Lupinus and Lens. Developing efficient analytical approaches for profiling their bioactive matrix is a prerequisite to provide proof for their health benefits or nutritive traits. Eight Lupinus and Lens seed accessions were subjected to liquid chromatography-mass spectrometry (UPLC-MS)-based metabolomic study, which identified 66 metabolites, viz. flavonoids, alkaloids, saponins, phenolics, fatty acids and sphingolipids. Chemometric tools were explored to assess heterogeneity across the two genera leading to elucidation of the species-most enriched and differential metabolites. The two dark-colored lentil cultivars are identified as the richest source of functional foods with presumed therapeutic benefits; however, Lupinus hispanicus was proved to be the most nutritive accession. To our knowledge, this study provides the first UPLC-MS-based comparative metabolite profiling of Lupinus and Lens seeds. This platform was also able to discern metabolites diversity at the intraspecific level among Lupinus species and Lens cultivars. |
| Databáze: | OpenAIRE |
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