Intrinsic radiosensitivity and repair of sublethal radiation-induced damage in canine osteosarcoma cell lines
| Autor: | Christopher G. Morris, David M. Lurie, Courtney L Fitzpatrick, Francis J. Bova, Rowan J. Milner, Marc E. Salute, James P. Farese, Dietmar W. Siemann, Didier A. Rajon |
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| Rok vydání: | 2008 |
| Předmět: |
Time Factors
Cell Survival medicine.medical_treatment Bone Neoplasms Radiation induced Linear quadratic Biology Radiation Tolerance Canine Osteosarcoma Dogs Cell Line Tumor medicine Animals Dog Diseases Radiosensitivity Clonogenic assay Osteosarcoma General Veterinary business.industry Dose-Response Relationship Radiation General Medicine Radiation therapy Dose–response relationship Cell culture Cancer research Nuclear medicine business |
| Zdroj: | American Journal of Veterinary Research. 69:1197-1202 |
| ISSN: | 0002-9645 |
| Popis: | Objective—To characterize the radiosensitivity and capacity for sublethal damage repair (SLDR) of radiation-induced injury in 4 canine osteosarcoma cell lines. Sample Population—4 canine osteosarcoma cell lines (HMPOS, POS, COS 31, and D17). Procedures—A clonogenic colony-forming assay was used to evaluate the cell lines' intrinsic radiosensitivities and SLDR capacities. Dose-response curves for the cell lines were generated by fitting the surviving fractions after radiation doses of 0 (control cells), 1, 2, 3, 6, and 9 Gy to a linear quadratic model. To evaluate SLDR, cell lines were exposed to 2 doses of 3 Gy (split-dose experiments) at an interval of 0 (single 6-Gy dose), 2, 4, 6, or 24 hours, after which the surviving fractions were assessed. Results—Mean surviving fraction did not differ significantly among the 4 cell lines at the radiation doses tested. Mean surviving fraction at 2 Gy was high (0.62), and the α/β ratios (predictor of tissue sensitivity to radiation therapy) for the cell lines were low (mean ratio, 3.47). The split-dose experiments revealed a 2.8- to 3.9-fold increase in cell survival when the radiation doses were applied at an interval of 24 hours, compared with cell survival after radiation doses were applied consecutively (0-hour interval). Conclusions and Clinical Relevance—Results indicated that these canine osteosarcoma cell lines are fairly radioresistant; α/β ratios were similar to those of nonneoplastic, lateresponding tissues. Future clinical investigations should involve increasing the fraction size in a manner that maximizes tumor killing without adverse effects on the nonneoplastic surrounding tissues. |
| Databáze: | OpenAIRE |
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