Characterization of an HIV-1 p24gag epitope recognized by a CD8+ cytotoxic T-cell clone
| Autor: | Florence Buseyne, Stefan Stevanovic, Yves Rivière, Hans-Georg Rammensee |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Immunology
Cell HIV Core Protein p24 Clone (cell biology) Peptide CD8-Positive T-Lymphocytes Epitope Major Histocompatibility Complex Epitopes HLA-C medicine Humans Immunology and Allergy Cytotoxic T cell Alleles Cells Cultured chemistry.chemical_classification Chemistry Histocompatibility Antigens Class I Virology Molecular biology Clone Cells Amino acid medicine.anatomical_structure HIV-1 Oligopeptides Epitope Mapping CD8 T-Lymphocytes Cytotoxic |
| Zdroj: | Immunology Letters. 55:145-149 |
| ISSN: | 0165-2478 |
| Popis: | A CD8 + cytotoxic T-cell clone that recognized HIV p24 gag was isolated from an infected individual. The minimal epitope was localized to amino acids 308–316 (QASQEVKNW). Using allogeneic target cells, we found that lysis was restricted by the HLA-Cw0401 molecule. We observed that C1R cells, that express the HLA-Cw0401 allele are able to present the peptide to the cytotoxic clone, but with reduced efficiency. Other B-cell lines, that have been genotyped as HLA-Cw0401 + were unable to present the peptide to the clone, suggesting the existence of other variants of HLA-Cw0401 or a loss of cell surface expression of this molecule. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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