Colono-protective Potentiality of Methanolic Bark Extract of Acacia catechu: A Medicinal Plant against 1,2-Dimethylhydrazine-Induced Toxicity in Wistar Rats
| Autor: | Syed Kazim Hasan, Preeti Barnwal, Sarwat Sultana, Rahat Ali, Abul Vafa, Aisha Siddiqi, Alpa Shree, Shekh Mohammad Afzal, Johirul Islam |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Antioxidant Colon Health Toxicology and Mutagenesis medicine.medical_treatment Glutathione reductase Toxicology medicine.disease_cause Protective Agents Drug Administration Schedule Pathology and Forensic Medicine Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound Random Allocation 0302 clinical medicine medicine Animals Rats Wistar chemistry.chemical_classification Inflammation Plants Medicinal Traditional medicine Chemistry Plant Extracts Glutathione peroxidase Methanol Acacia General Medicine Glutathione 1 2-Dimethylhydrazine Rats Oxidative Stress 030104 developmental biology 030220 oncology & carcinogenesis Toxicity Carcinogens Plant Bark Female Oxidative stress Biomarkers Phytotherapy |
| Zdroj: | Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer. 37(4) |
| ISSN: | 2162-6537 |
| Popis: | The protective efficacy of methanolic bark extract of Acacia catechu Willd. (MEBA) against 1,2-dimethylhydrazine (DMH)-induced colon toxicity was investigated. Acacia catechu is considered one of the most potent medicines for various diseases in Ayurveda, a traditional system of Indian medicine. It is a widely used herb that contains a variety of bioactive components such as phenolic acids, alkaloids, and flavonoids among others. In the present study, MEBA was used as a pretreatment orally at two doses (250 and 500 mg/kg body weight [b.w.] once daily for 7 days), and DMH was administered (at a dose of 40 mg/kg b.w.) subcutaneously on day 7 in Wistar rats. The protective potential of MEBA was assessed in terms of the activity of antioxidant enzymes, lipid peroxidation, and expression of inflammatory markers (iNOS, COX-2, NF-κB, IL-6). Pretreatment with MEBA significantly abrogated oxidative damage by diminishing tissue lipid peroxidation, increasing enzymatic activities of various antioxidant enzymes (catalase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, reduced glutathione), and diminishing the induced expression of inflammatory markers in the colon tissue of Wistar rats. Furthermore, histopathological findings revealed that pretreatment with (MEBA) reduced intense filtration of inflammatory cells and significantly restored the architecture of colonic tissue. The results of this study indicate that MEBA significantly suppresses DMH-induced toxicity by ameliorating oxidative stress and inflammation and by restoring the architecture of colon tissue. |
| Databáze: | OpenAIRE |
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