Advancement of reproductive senescence and changes in the early expression of estrogen, progesterone and micro-opioid receptors induced by neonatal hypoxia in the female rat
| Autor: | Marcelo Ezquer, Graciela A. Jahn, Alicia M. Seltzer, Susana Ruth Valdez |
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| Rok vydání: | 2008 |
| Předmět: |
Senescence
medicine.medical_specialty medicine.drug_class Receptors Opioid mu Biology Functional Laterality Rats Sprague-Dawley Reproductive senescence Ischemia Internal medicine medicine Animals RNA Messenger Receptor Hypoxia Molecular Biology Progesterone Estrous cycle Analysis of Variance General Neuroscience Reproduction Age Factors Gene Expression Regulation Developmental Estrogens Hypoxia (medical) Gonadotropin secretion Rats Endocrinology Animals Newborn Estrogen Hormone receptor Female Neurology (clinical) medicine.symptom Developmental Biology |
| Zdroj: | Brain research. 1214 |
| ISSN: | 0006-8993 |
| Popis: | Perinatal hypoxia is a frequent birth complication, and although its early consequences on brain development have been well studied, few studies address any long-term effects. Postnatal insults producing small disturbances in endocrine function can have marked and long-lasting effects. In the present work we studied the effects of two types of perinatal brain injury: global hypoxia (H, 6.5% O2 for 50 min) and hypoxia plus ischemia (HI, ligature of the right carotid artery) on female rat reproductive performance and expression of mediobasal hypothalamus-preoptic area (MBH-PO) estrogen, progesterone and micro-opioid receptors at different times after injury, measuring the mRNA (by semiquantitative RT-PCR) and protein (by Western blot). H or HI advanced approximately 3 months after the appearance of blunted preovulatory LH surges and cyclic irregularities (prolonged estrus) characteristic of the early stages of reproductive senescence. 48 h after H or HI we observed decreases in ERbeta, microOR and PR (only in the H group) mRNAs and in total ER and microOR proteins, followed by increased PR levels (mRNA and protein) 7 days post-injury and by increased microOR protein and ERbeta mRNA in the H group and ERalpha, ERbeta and microOR mRNAs and ER protein in the HI group 30 days post-injury. Thus, an episode of hypoxia suffered during early postnatal life induces premature reproductive senescence on the female rats, accompanied by early changes in some MBH-PO hormone receptors (microOR, ER and PR), whose expression is intimately involved in the regulation of gonadotropin secretion and female sexual cyclicity. |
| Databáze: | OpenAIRE |
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