SYNTHESIS, CHARACTERIZATION AND APPLICATION OF A POLYURETHANE-BASED SUPPORT FOR IMMOBILIZING MEMBRANE-BOUND LIPASE
| Autor: | Grazielle Santos Silva Andrade, H. F. de Castro, A. L. L. Rico, Pedro Oliveira, M. S. Soares |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Polyurethane General Chemical Engineering 01 natural sciences chemistry.chemical_compound Hydrolysis Polyol 010608 biotechnology Mucor circinelloides lcsh:Chemical engineering Lipase chemistry.chemical_classification biology 010405 organic chemistry lcsh:TP155-156 Transesterification Polymer biology.organism_classification Babassu oil 0104 chemical sciences chemistry Chemical engineering biology.protein |
| Zdroj: | Brazilian Journal of Chemical Engineering, Vol 34, Iss 1, Pp 29-39 Brazilian Journal of Chemical Engineering, Volume: 34, Issue: 1, Pages: 29-39, Published: JAN 2017 Brazilian Journal of Chemical Engineering v.34 n.1 2017 Brazilian Journal of Chemical Engineering Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
| ISSN: | 0104-6632 |
| DOI: | 10.1590/0104-6632.20170341s20140227 |
| Popis: | This study conducted an assessment of polyurethane foams that were synthesized by one-shot process and used as a low-cost support to immobilize Mucor circinelloides URM 4182 whole-cells presenting high lipolytic activity. Polyols with different molecular weights (1100 to 6000 g mol-1) were applied to synthesize the polymer matrix, and the agitation speed effect was used for controlling the average pore size of the investigated polyurethane foams. The physical and mechanical properties of the polymers were evaluated by standard test methods, and their morphology was identified by Scanning Electron Microscopy. The immobilization procedure efficiency was assessed by quantifying the capability of the matrices to attach the cells and the catalytic activity of the biocatalysts in both aqueous (olive oil hydrolysis) and non-aqueous media (ethanolysis of babassu oil) under single and consecutive batch runs. Although all synthesized matrices were suitable to immobilize the whole cells with high catalytic performance, a better set of parameters was attained when the polyol ether with molecular weight of 6000 g mol-1 and 1100 g mol-1 was used. Both matrices yielded immobilized biocatalysts with high hydrolysis and transesterification activities, and exhibited a satisfactory operational stability with 96% and 81% retention of their initial hydrolytic and transesterification activities after three consecutive batch runs. |
| Databáze: | OpenAIRE |
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