A subset of low density granulocytes is associated with vascular calcification in chronic kidney disease patients

Autor: Javier Rodríguez-Carrio, Catalina Ulloa, Carmen Díaz-Corte, Mariana Seijo, Adriana S. Dusso, Carmen Rodriguez-Suarez, Natalia Carrillo-López, Jorge B. Cannata-Andía, Minerva Rodríguez-García, Ana Suárez
Rok vydání: 2019
Předmět:
0301 basic medicine
CD31
Male
Neutrophils
medicine.medical_treatment
kidney disease
lcsh:Medicine
Ciencias de la Salud
Monocytes
Pathogenesis
0302 clinical medicine
lcsh:Science
Multidisciplinary
Kidney diseases
CD68
Middle Aged
Otras Ciencias de la Salud
purl.org/becyt/ford/3 [https]
Female
medicine.symptom
Adult
CIENCIAS MÉDICAS Y DE LA SALUD
Inflammation
Peripheral blood mononuclear cell
Granulopoiesis
Article
Peritoneal dialysis
purl.org/becyt/ford/3.3 [https]
03 medical and health sciences
Young Adult
medicine
Humans
Renal Insufficiency
Chronic
Vascular Calcification
Aged
business.industry
lcsh:R
Diagnostic markers
Translational research
medicine.disease
030104 developmental biology
traslational research
diagnostic markers
Immunology
Leukocytes
Mononuclear
lcsh:Q
business
Biomarkers
030215 immunology
Kidney disease
Granulocytes
Zdroj: Scientific Reports
Scopus
RUO. Repositorio Institucional de la Universidad de Oviedo
instname
Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
ISSN: 2045-2322
Popis: Inflammation is central to chronic kidney disease (CKD) pathogenesis and vascular outcomes, but the exact players remain unidentified. Since low density granulocytes (LDGs) are emerging mediators in inflammatory conditions, we aimed to evaluate whether LDGs may be altered in CKD and related to clinical outcomes as biomarkers. To his end, LDGs subsets were measured in peripheral blood by flow cytometry and confocal microscopy in 33 CKD patients undergoing peritoneal dialysis and 15 healthy controls (HC). Analyses were replicated in an additional cohort. DEF3 (marker of early granulopoiesis) gene expression on PBMCs was quantified by qPCR. Total CD15+ LDGs and both CD14lowCD16+ and CD14−CD16− subsets were expanded in CKD. The relative frequency of the CD14−CD16− subpopulation was higher among the CD15+ pool in CKD. This alteration was stable over-time. The increased CD14−CD16−CD15+ paralleled Kauppila scores and DEF3 expression, whereas no association was found with CD14lowCD16+ CD15+. Both subsets differed in their CD11b, CD10, CD35, CD31, CD62L, IFNAR1 and CD68 expression, FSC/SSC features and nuclear morphology, pointing to different origins and maturation status. In conclusion, LDGs were expanded in CKD showing a skewed distribution towards a CD14−CD16−CD15+ enrichment, in association with vascular calcification. DEF3 expression in PBMC can be a marker of LDG expansion. Fil: Rodríguez Carrio, Javier. Hospital Universitario Central de Asturias. Instituto de Investigación Sanitaria del Principado de Asturias (ISPA). Bone and Mineral Research Unit; España. Universidad de Oviedo; España Fil: Carrillo López, Natalia. Hospital Universitario Central de Asturias; España Fil: Ulloa, Catalina. Hospital Universitario Central de Asturias; España Fil: Seijo, Mariana. Hospital Universitario Central de Asturias; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina Fil: Rodríguez García, Minerva. Hospital Universitario Central de Asturias; España Fil: Rodríguez Suárez, Carmen. Hospital Universitario Central de Asturias; España Fil: Díaz-Corte, Carmen. Hospital Universitario Central de Asturias; España Fil: Cannata Andía, Jorge B.. Universidad de Oviedo; España. Hospital Universitario Central de Asturias; España Fil: Suárez, Ana. Universidad de Oviedo; España Fil: Dusso, Adriana. Hospital Universitario Central de Asturias; España
Databáze: OpenAIRE
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