The regenerative response of cardiac interstitial cells
| Autor: | Chris Jopling, Laura Rolland, Laurent Gamba, Dany Severac, Alenca Harrington, Thomas Moore-Morris, Marine Pratlong, Adèle Faucherre, Girisaran Gangatharan |
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| Přispěvatelé: | Institut de Génomique Fonctionnelle (IGF), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), BioCampus (BCM), Faucherre, Adèle |
| Rok vydání: | 2021 |
| Předmět: |
biology
[SDV]Life Sciences [q-bio] Transgene Cell type specific cardiac regeneration [SDV.BDD.MOR]Life Sciences [q-bio]/Development Biology/Morphogenesis scRNAseq biology.organism_classification Interstitial cell [SDV.BDD.MOR] Life Sciences [q-bio]/Development Biology/Morphogenesis Cell biology [SDV] Life Sciences [q-bio] Cardiac regeneration [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Chemical inhibition Zebrafish |
| DOI: | 10.1101/2021.10.25.465720 |
| Popis: | Understanding how certain animals are capable of regenerating their hearts will provide much needed insights into how this process can be induced in humans in order to reverse the damage caused by myocardial infarction. Currently, it is becoming increasingly evident that cardiac interstitial cells play crucial roles during cardiac regeneration. To understand how interstitial cells behave during cardiac regeneration, we performed single-cell RNA sequencing (scRNA-seq) of regenerating zebrafish hearts. Using a combination of immunohistochemistry, chemical inhibition and novel transgenic animals, we were able to investigate the role of cell type specific responses during cardiac regeneration. This approach allowed us to identify a number of important regenerative mechanisms within the interstitial cell populations. Here, we provide here a detailed insight into how interstitial cells behave during cardiac regeneration and identify a number of novel features of these cells which will serve to increase our understanding of how this process could eventually be induced in humans. |
| Databáze: | OpenAIRE |
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