Determination of total intracellular lipid content by flow cytofluorometry
| Autor: | V.N. Smirnov, D. N. Mukhin, S.A. Rudchenko, Vladimir V. Tertov, L.D. Bergelson, Yu. G. Molotkovsky, Alexander N. Orekhov |
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| Rok vydání: | 1985 |
| Předmět: |
Intracellular Fluid
In situ Biophysics Biology Biochemistry Mice chemistry.chemical_compound Glycerol Animals Humans Molecular Biology Cells Cultured Triglycerides Fluorescent Dyes Chromatography Triglyceride Cholesterol Cell Biology Fibroblasts Embryo Mammalian Flow Cytometry Lipids Fluorescence Body Fluids Rats chemistry Lipid content lipids (amino acids peptides and proteins) Flow Cytofluorometry Intracellular |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(85)91067-8 |
| Popis: | It was demonstrated that perylenoyl-labeled triglyceride, rac-1,2-dioleoyl-3-[9(3-perylenoyl)nonanoyl]glycerol (PLTG), specifically stains the intracellular lipids in formalin-fixed and non-fixed cells in situ and in suspension. The fluorescence of labeled cells was registered on a FACS-II cytofluorometer. It was found that the mean intensity of fluorescence in PLTG-labeled cells of various types, both fixed and non-fixed, correlated with their total lipid content. There was no correlation, however, between the mean intensity of fluorescence and intracellular levels of lipids belonging to different classes: phospholipids, triglycerides, cholesterol or cholesteryl esters. |
| Databáze: | OpenAIRE |
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