βcatenin is a marker of poor clinical characteristics and suppressed immune infiltration in testicular germ cell tumors
Autor: | Michal Mego, Katarina Machalekova, Daniela Svetlovska, Katarina Rejlekova, Stanislav Spanik, Viera Miskovska, Michal Chovanec, Jozef Mardiak, Katarina Kalavska, Karol Kajo, Pavel Babal, Dusan Macak, Zuzana Cierna |
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Rok vydání: | 2018 |
Předmět: |
Male
0301 basic medicine Cancer Research Lymphocyte B7-H1 Antigen 0302 clinical medicine Surgical oncology Tumor Microenvironment Medicine WNTβ pathway Wnt Signaling Pathway beta Catenin biology Wnt signaling pathway Middle Aged Neoplasms Germ Cell and Embryonal Prognosis lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Immunohistochemistry Tumor infiltrating lymphocytes medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Research Article Adult PD-L1 Adolescent Models Biological lcsh:RC254-282 Young Adult βcatenin 03 medical and health sciences Lymphocytes Tumor-Infiltrating Immune system Testicular Neoplasms Biomarkers Tumor Genetics Humans Aged Neoplasm Staging business.industry Tumor-infiltrating lymphocytes Systemic-immune inflammation medicine.disease 030104 developmental biology biology.protein Cancer research Germ cell tumors business |
Zdroj: | BMC Cancer, Vol 18, Iss 1, Pp 1-10 (2018) BMC Cancer |
ISSN: | 1471-2407 |
Popis: | Background WNT/βcatenin (WNTβ) pathway is activated in early stages of embryonic development. We aimed to evaluate the significance of βcatenin in germ cell tumors (GCTs) and explore associations with the inflamed environment. Methods Surgical specimens from 247 patients were analyzed. Βcatenin expression was detected in the tumor tissue by immunohistochemistry and correlated with clinical characteristics, outcome, PD-L1 expression and systemic immune-inflammation index (SII). The Ingenuity Pathway Analysis (IPA) was used to investigate the immune-cell related effects of βcatenin and PD-L1 encoding genes. Results βcatenin was expressed in 86.2% of GCTs. The expression in seminomas was significantly lower compared to all subtypes of non-seminoma (all P 150) was associated with primary mediastinal non-seminoma (P = 0.035), intermediate/poor risk disease (P = 0.033) and high tumor markers (P = 0.035). We observed a positive correlation with the PD-L1 in tumor and an inverse correlation with the SII. IPA uncovered relationships of CTNNB (βcatenin) and CD274 (PD-L1) genes and their effects on differentiation, proliferation and activation of lymphocyte subtypes. Conclusion Herein, we showed that βcatenin is associated with male adult GCT characteristics as well as supressed immune environment. Electronic supplementary material The online version of this article (10.1186/s12885-018-4929-x) contains supplementary material, which is available to authorized users. |
Databáze: | OpenAIRE |
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