βcatenin is a marker of poor clinical characteristics and suppressed immune infiltration in testicular germ cell tumors

Autor: Michal Mego, Katarina Machalekova, Daniela Svetlovska, Katarina Rejlekova, Stanislav Spanik, Viera Miskovska, Michal Chovanec, Jozef Mardiak, Katarina Kalavska, Karol Kajo, Pavel Babal, Dusan Macak, Zuzana Cierna
Rok vydání: 2018
Předmět:
Male
0301 basic medicine
Cancer Research
Lymphocyte
B7-H1 Antigen
0302 clinical medicine
Surgical oncology
Tumor Microenvironment
Medicine
WNTβ pathway
Wnt Signaling Pathway
beta Catenin
biology
Wnt signaling pathway
Middle Aged
Neoplasms
Germ Cell and Embryonal
Prognosis
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Immunohistochemistry
Tumor infiltrating lymphocytes
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Research Article
Adult
PD-L1
Adolescent
Models
Biological
lcsh:RC254-282
Young Adult
βcatenin
03 medical and health sciences
Lymphocytes
Tumor-Infiltrating
Immune system
Testicular Neoplasms
Biomarkers
Tumor
Genetics
Humans
Aged
Neoplasm Staging
business.industry
Tumor-infiltrating lymphocytes
Systemic-immune inflammation
medicine.disease
030104 developmental biology
biology.protein
Cancer research
Germ cell tumors
business
Zdroj: BMC Cancer, Vol 18, Iss 1, Pp 1-10 (2018)
BMC Cancer
ISSN: 1471-2407
Popis: Background WNT/βcatenin (WNTβ) pathway is activated in early stages of embryonic development. We aimed to evaluate the significance of βcatenin in germ cell tumors (GCTs) and explore associations with the inflamed environment. Methods Surgical specimens from 247 patients were analyzed. Βcatenin expression was detected in the tumor tissue by immunohistochemistry and correlated with clinical characteristics, outcome, PD-L1 expression and systemic immune-inflammation index (SII). The Ingenuity Pathway Analysis (IPA) was used to investigate the immune-cell related effects of βcatenin and PD-L1 encoding genes. Results βcatenin was expressed in 86.2% of GCTs. The expression in seminomas was significantly lower compared to all subtypes of non-seminoma (all P 150) was associated with primary mediastinal non-seminoma (P = 0.035), intermediate/poor risk disease (P = 0.033) and high tumor markers (P = 0.035). We observed a positive correlation with the PD-L1 in tumor and an inverse correlation with the SII. IPA uncovered relationships of CTNNB (βcatenin) and CD274 (PD-L1) genes and their effects on differentiation, proliferation and activation of lymphocyte subtypes. Conclusion Herein, we showed that βcatenin is associated with male adult GCT characteristics as well as supressed immune environment. Electronic supplementary material The online version of this article (10.1186/s12885-018-4929-x) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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