Picogram detection of metal ions by melanin-sensitized piezoelectric sensor
| Autor: | Meng-Yen Hong, Meng-Te Wang, Chia-Wei Su, G. Steven Huang, Yu-Shiun Chen |
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| Rok vydání: | 2006 |
| Předmět: |
Detection limit
Melanins Circular dichroism Spectrophotometry Infrared Chemistry Metal ions in aqueous solution Circular Dichroism Biomedical Engineering Biophysics Analytical chemistry Infrared spectroscopy General Medicine Quartz crystal microbalance Biosensing Techniques Quartz Sensitivity and Specificity Metal Metals visual_art Electrode Electrochemistry visual_art.visual_art_medium Thin film Crystallization Biotechnology |
| Zdroj: | Biosensorsbioelectronics. 23(3) |
| ISSN: | 0956-5663 |
| Popis: | A heavy metal ion sensor was constructed by cross-linking melanin onto the gold electrode of quartz crystal microbalance (QCM). A mercury ion sensitivity of 518+/-37 Hz/ppm was observed, a substantial increase in sensitivity compared to previous reports of 10-50 Hz/ppm with the limit of detection at 5 ppb. Detection of other metal ions including Sn(2+), Ge(4+), Li(+), Zn(2+), Cu(2+), Bi(3+), Co(2+), Al(3+), Ni(2+), Ag(+), and Fe(3+) were also performed. Unexpectedly, binding of Mn(7+), Pb(2+), Cd(2+), and Cr(3+) increased resonant frequencies. The surface profile of melanin thin film upon binding to metal ions was investigated by atomic force microscopy (AFM). Structural change of melanin upon binding to metal ions was characterized by circular dichroism and by infrared spectroscopy. The current study provides the first example of melanin-coated piezoelectric sensor showing high sensitivity and selectivity to metal ions. |
| Databáze: | OpenAIRE |
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