Telomerase expression marks transitional growth-associated skeletal progenitor/stem cells
| Autor: | Diana L. Carlone, Rebecca D. Riba‐Wolman, Alessio Tovaglieri, Rudolf Jaenisch, Dana M. Ambruzs, Benjamin E. Mead, Lijie Jiang, David T. Breault, Christopher J. Lengner, Luke T. Deary, Manasvi S. Shah |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Telomerase mTert Population Biology telomerase 03 medical and health sciences Mice pluripotent 0302 clinical medicine Bone Marrow medicine Animals Progenitor cell education Cellular Senescence Cell Proliferation Bone growth education.field_of_study Stem Cells Mesenchymal stem cell Cell Cycle Epithelial Cells Cell Biology Endochondral bone growth Cell biology Tissue‐specific Stem Cells 030104 developmental biology medicine.anatomical_structure skeletal stem cells Molecular Medicine Bone marrow Stem cell postnatal bone growth 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Stem Cells (Dayton, Ohio) |
| ISSN: | 1549-4918 |
| Popis: | Skeletal progenitor/stem cells (SSCs) play a critical role in postnatal bone growth and maintenance. Telomerase (Tert) activity prevents cellular senescence and is required for maintenance of stem cells in self‐renewing tissues. Here we investigated the role of mTert‐expressing cells in postnatal mouse long bone and found that mTert expression is enriched at the time of adolescent bone growth. mTert‐GFP+ cells were identified in regions known to house SSCs, including the metaphyseal stroma, growth plate, and the bone marrow. We also show that mTert‐expressing cells are a distinct SSC population with enriched colony‐forming capacity and contribute to multiple mesenchymal lineages, in vitro. In contrast, in vivo lineage‐tracing studies identified mTert + cells as osteochondral progenitors and contribute to the bone‐forming cell pool during endochondral bone growth with a subset persisting into adulthood. Taken together, our results show that mTert expression is temporally regulated and marks SSCs during a discrete phase of transitional growth between rapid bone growth and maintenance. In long bones, osteochondral progenitor cells function during rapid bone growth while osteoadipogenic progenitors act during bone maintenance. We showed that mTert is expressed in an age‐dependent manner and marks growth‐associated osteochondral progenitor cells during transitional growth, a discrete time period between rapid bone growth and bone maintenance. |
| Databáze: | OpenAIRE |
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