Host specificity in the diversity and transfer of lsa resistance genes in group B Streptococcus
| Autor: | Pierre-Emmanuel Douarre, Claire Poyart, Elisabeth Sauvage, Philippe Glaser |
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| Přispěvatelé: | Biologie des Bactéries pathogènes à Gram-positif - Biology of Gram-Positive Pathogens, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Centre national de Référence des Streptocoques (CNR), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), This work was supported by the French National Research Agency (grant 2010-PATH-004-02) and the LabEx project IBEID., ANR-10-PATH-0004,MobileGenomics(2010), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), BOUYSSIE, Reine, Programme transnational sur les agents infectieux - - MobileGenomics2010 - ANR-10-PATH-0004 - Pathogenomics - VALID, Integrative Biology of Emerging Infectious Diseases - - IBEID2010 - ANR-10-LABX-0062 - LABX - VALID, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
MESH: Sequence Analysis
DNA MESH: Diterpenes [SDV]Life Sciences [q-bio] Sequence Homology Genome chemistry.chemical_compound Gene cluster MESH: Cattle Diseases Pharmacology (medical) MESH: Animals MESH: Genetic Variation MESH: Sequence Homology Recombination Genetic Genetics Streptogramin A 0303 health sciences MESH: Microbial Sensitivity Tests Lincosamides Anti-Bacterial Agents [SDV] Life Sciences [q-bio] MESH: Cattle Infectious Diseases Chromosomal region Horizontal gene transfer MESH: Recombination Genetic Diterpenes MESH: Genes Bacterial Microbiology (medical) Gene Transfer Horizontal MESH: Host Specificity medicine.drug_class Cattle Diseases Microbial Sensitivity Tests Biology Host Specificity Streptococcus agalactiae 03 medical and health sciences MESH: Polycyclic Compounds MESH: Anti-Bacterial Agents Drug Resistance Bacterial MESH: Streptogramin A MESH: Drug Resistance Bacterial medicine Animals Polycyclic Compounds MESH: Lincosamides Gene Gram-Positive Bacterial Infections 030304 developmental biology Pharmacology Comparative genomics 030306 microbiology Genetic Variation Sequence Analysis DNA bacterial infections and mycoses MESH: Streptococcus agalactiae MESH: Gene Transfer Horizontal chemistry Genes Bacterial bacteria Cattle MESH: Gram-Positive Bacterial Infections |
| Zdroj: | Journal of Antimicrobial Chemotherapy Journal of Antimicrobial Chemotherapy, Oxford University Press (OUP), 2015, 70 (12), pp.3205-13. ⟨10.1093/jac/dkv277⟩ Journal of Antimicrobial Chemotherapy, 2015, 70 (12), pp.3205-13. ⟨10.1093/jac/dkv277⟩ |
| ISSN: | 0305-7453 1460-2091 |
| DOI: | 10.1093/jac/dkv277⟩ |
| Popis: | OBJECTIVES In group B Streptococcus (GBS), cross-resistance to lincosamides, streptogramin A and pleuromutilins (LSAP) is mediated by the acquisition of lsa genes. Here, we characterized the diversity, mobility and ecology of lsa genes in this species. METHODS lsa variants were systematically identified by BLAST searches in the genomes of 531 GBS strains from different hosts and geographical origins. The associated phenotypes were determined by a microdilution MIC method. Acquisition of resistance genes was deduced from comparative genomics and phylogeny. Their mobility was tested by conjugation experiments. RESULTS lsa(E) and three variants of lsa(C) were identified in GBS strains. Two lsa(C) variants had not been previously reported. All four variants conferred LSAP phenotypes. lsa(E) was located in a multiresistance gene cluster of a single human strain. This gene was transferred by a high-frequency recombination-type mechanism between GBS strains. Two lsa(C) variants are carried in six unrelated human strains by two similar elements specifically integrated in the oriT site of four different classes of integrative and conjugative elements (ICEs). Strikingly, the acquisition of the resistance gene always occurred by the integration of the element into a resident ICE. The third lsa(C) variant was located at the same site in the core genome of 11 genetically distant bovine strains and was likely propagated by horizontal transfer of the corresponding chromosomal region. CONCLUSIONS lsa genes in GBS show distinct host specificities and modes of transfer. In general, their dissemination is mediated by recombination rather than by the transfer of conjugative elements. |
| Databáze: | OpenAIRE |
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