Best practices in cell culture: an overview
| Autor: | Lia Campbell, Gertrude C. Buehring, Frank P. Simione, Raymond W. Nims, Yvonne Reid, John W. Harbell, Paul J. Price, John M. Baust, Elmore E |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Receipt Cryopreservation business.industry Best practice Cell Culture Techniques Target audience Cell Biology General Medicine Public relations Unmet needs Cell Line Culture Media 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Cell culture 030220 oncology & carcinogenesis Animals business Developmental Biology |
| Zdroj: | In vitro cellulardevelopmental biology. Animal. 53(8) |
| ISSN: | 1543-706X |
| Popis: | This overview describes a series of articles to provide an unmet need for information on best practices in animal cell culture. The target audience primarily consists of entry-level scientists with minimal experience in cell culture. It also include scientists, journalists, and educators with some experience in cell culture, but in need of a refresher in best practices. The articles will be published in this journal over a six-month period and will emphasize best practices in: (1) media selection; (2) use and evaluation of animal serum as a component of cell culture medium; (3) receipt of new cells into the laboratory; (4) naming cell lines; (5) authenticating cell line identity; (6) detecting and mitigating risk of cell culture contamination; (7) cryopreservation and thawing of cells; and (8) storing and shipping viable cells. |
| Databáze: | OpenAIRE |
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