Molecular screening of oral precancer
| Autor: | Ruud H. Brakenhoff, Boudewijn J.M. Braakhuis, C. René Leemans, Meindert Zwart, Isaäc van der Waal, A. Peggy Graveland, Elisabeth Bloemena, Paul Cobussen, Arjen Brink, Jantine F. Bremmer, Michiel de Maaker |
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| Přispěvatelé: | MKA Vumc (OII, ACTA), Maxillofacial Surgery (VUmc), CCA - Oncogenesis, Otolaryngology / Head & Neck Surgery, Radiation Oncology, Pathology, Oral and Maxillofacial Surgery / Oral Pathology |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Adult
Genetic Markers Male Cancer Research medicine.medical_specialty Pathology Histopathological grading Gastroenterology Sensitivity and Specificity Malignant transformation Loss of heterozygosity SDG 3 - Good Health and Well-being Risk Factors Internal medicine Biopsy medicine Humans Mass Screening Early Detection of Cancer Leukoplakia Aged Aged 80 and over Molecular screening medicine.diagnostic_test business.industry Cancer Middle Aged medicine.disease stomatognathic diseases Oncology Mutation Immunohistochemistry Female Oral Surgery Leukoplakia Oral business Microsatellite Repeats |
| Zdroj: | Graveland, A P, Bremmer, J F, de Maaker, M, Brink, A, Cobussen, P, Zwart, M, Braakhuis, B J M, Bloemena, E, van der Waal, I, Leemans, C R & Brakenhoff, R H 2013, ' Molecular screening of oral precancer ', Oral Oncology, vol. 49, no. 12, pp. 1129-1135 . https://doi.org/10.1016/j.oraloncology.2013.09.005 Oral Oncology, 49(12), 1129-1135. Elsevier Oral Oncology, 49(12), 1129-1135. Elsevier Limited |
| ISSN: | 1368-8375 |
| DOI: | 10.1016/j.oraloncology.2013.09.005 |
| Popis: | Objectives: Early detection and treatment of high risk premalignant mucosal changes of the oral cavity, will expectedly improve survival and reduce treatment-related morbidity. Aims of this study were to evaluate a non-invasive screening approach and to assess the value of molecular markers to identify patients at risk for oral cancer. Materials and Methods: Exfoliated cells and biopsies were obtained from oral leukoplakia lesions of 43 patients, of whom six developed oral cancer. All samples were investigated for loss of heterozygosity (LOH) at chromosomes 3p, 9p, 11q and 17p using microsatellite markers. On the biopsy specimen additional immunohistochemical staining for p53, TP53 mutation analysis and histopathological grading were performed. Results: The analytical sensitivity of the non-invasive assay using exfoliated cells to detect genetic changes present in the lesions was 45% (9 of 20), the specificity was 100% (19 of 19), and the positive predictive value was also 100% (9 of 9). LOH was present in 20 of 39 (51%) of the biopsies with uniformly LOH at 9p. Mutated TP53 and LOH at 9p in the biopsy, as single markers and in combination, were significant risk factors for malignant progression of leukoplakia to oral cancer (Kaplan-Meier analysis, p |
| Databáze: | OpenAIRE |
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