A Novel Actin-bundling Kinesin-related Protein from Dictyostelium discoideum
| Autor: | Kazuo Sutoh, Atsushi Ishiji, Issei Mabuchi, Sosuke Iwai |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Recombinant Fusion Proteins
Genes Protozoan Molecular Sequence Data Protozoan Proteins Kinesins Arp2/3 complex macromolecular substances Biology Filamin Microtubules Biochemistry Animals Dictyostelium Amino Acid Sequence Actin-binding protein Cytoskeleton Molecular Biology Molecular Motor Proteins Microfilament Proteins Actin remodeling Cell Biology Actins Peptide Fragments Protein Structure Tertiary Cell biology Treadmilling Formins biology.protein MDia1 |
| Zdroj: | Journal of Biological Chemistry. 279:4696-4704 |
| ISSN: | 0021-9258 |
| Popis: | Actin filaments and microtubules are two major cytoskeletal systems involved in wide cellular processes, and the organizations of their filamentous networks are regulated by a large number of associated proteins. Recently, evidence has accumulated for the functional cooperation between the two filament systems via associated proteins. However, little is known about the interactions of the kinesin superfamily proteins, a class of microtubule-based motor proteins, with actin filaments. Here, we describe the identification and characterization of a novel kinesin-related protein named DdKin5 from Dictyostelium. DdKin5 consists of an N-terminal conserved motor domain, a central stalk region, and a C-terminal tail domain. The motor domain showed binding to microtubules in an ATP-dependent manner that is characteristic of kinesin-related proteins. We found that the C-terminal tail domain directly interacts with actin filaments and bundles them in vitro. Immunofluorescence studies showed that DdKin5 is specifically enriched at the actin-rich surface protrusions in cells. Overexpression of the DdKin5 protein affected the organization of actin filaments in cells. We propose that a kinesin-related protein, DdKin5, is a novel actin-bundling protein and a potential cross-linker of actin filaments and microtubules associated with specific actin-based structures in Dictyostelium. |
| Databáze: | OpenAIRE |
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