Correction: Mitochondrial oxidative phosphorylation in peripheral blood mononuclear cells is decreased in chronic HIV and correlates with immune dysregulation
| Autor: | Cecilia M. Shikuma, Brooks I. Mitchell, Scott Bowler, Chathura Siriwardhana, Louie Mar A Gangcuangco, Dominic C. Chow, Mariana Gerschenson, Kalpana J. Kallianpur, Lindsay B Kohorn, Lishomwa C. Ndhlovu, Glen M. Chew |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
RNA viruses Male HIV Infections Signal transduction medicine.disease_cause Pathology and Laboratory Medicine Lymphocyte Activation Biochemistry Severity of Illness Index Monocytes Oxidative Phosphorylation White Blood Cells 0302 clinical medicine Immunodeficiency Viruses Animal Cells Cellular types Medicine and Health Sciences Medicine Didanosine Immune Response Energy-Producing Organelles Multidisciplinary Middle Aged Mitochondria Medical Microbiology Viral Pathogens Viruses Host-Pathogen Interactions RNA Viral Female medicine.symptom Cellular Structures and Organelles Pathogens Coreceptors medicine.drug Research Article Senescence medicine.medical_specialty Cell biology Blood cells Anti-HIV Agents CD14 Immune Cells Science Immunology T cells CD4-CD8 Ratio Inflammation Cytotoxic T cells Bioenergetics Peripheral blood mononuclear cell Microbiology Hawaii 03 medical and health sciences Zidovudine Signs and Symptoms Diagnostic Medicine Internal medicine Retroviruses Humans T Helper Cells Microbial Pathogens Biology and life sciences business.industry Lentivirus Organisms HIV Correction CD coreceptors Immune dysregulation 030104 developmental biology Endocrinology Cross-Sectional Studies HIV-1 Leukocytes Mononuclear business 030217 neurology & neurosurgery CD8 |
| Zdroj: | PLoS ONE, Vol 15, Iss 4, p e0231761 (2020) PLoS ONE PLoS ONE, Vol 16, Iss 3, p e0249428 (2021) |
| ISSN: | 1932-6203 |
| Popis: | BackgroundCellular immunometabolism among people living with HIV (PLWH) on antiretroviral therapy (ART) remains under investigated. We assessed the relationships between mitochondrial oxidative phosphorylation (OXPHOS) in peripheral blood mononuclear cells (PBMCs) and blood parameters associated with HIV immune dysregulation.MethodsPLWH ≥40 years old and on stable ART ≥3 months were enrolled (N = 149). OXPHOS complex I (CI, NADH dehydrogenase) and complex IV (CIV, cytochrome c oxidase) protein levels in PBMCs were quantified using immunoassays. Monocyte subsets and markers of T-cell activation, senescence, and exhaustion were measured on PBMC by flow cytometry. Plasma inflammatory mediators were quantified using a multiplex assay. HIV-uninfected group (N = 44) of similar age, gender, and ethnicity had available OXPHOS levels.ResultsPLWH had a median age of 51 years. Majority were male (88.6%), Caucasian (57.7%), and with undetectable plasma HIV RNA ConclusionCI PBMC protein levels were decreased in PLWH on ART. Decreased OXPHOS correlated with disease severity and inflammation. Further studies on the relationship between immunometabolism and immune dysregulation in HIV are warranted. |
| Databáze: | OpenAIRE |
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