Replication of double-stranded RNA of the virus-like particles in Saccharomyces cerevisiae
| Autor: | P A Sutherland, A M Newman, C S McLaughlin, R C Warner, S G Elliott |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Semiconservative replication
Immunology Cell Saccharomyces cerevisiae Population Centrifugation Isopycnic Virus Replication Microbiology Virology medicine RNA Viruses education RNA Double-Stranded education.field_of_study biology Cell Cycle RNA Cell cycle biology.organism_classification Molecular biology RNA silencing medicine.anatomical_structure Viral replication Insect Science Biophysics RNA Viral Research Article |
| Zdroj: | Journal of Virology. 38:263-271 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.38.1.263-271.1981 |
| Popis: | The mode of replication of the L double-stranded RNA (dsRNA) present in virus-like particles in Saccharomyces cerevisiae was examined by density transfer experiments. After transfer to light medium, significant amounts of fully heavy dsRNA persisted over a number of cell doublings. In addition, very little material of hybrid density was ever formed, and the accumulation of fully light material began as early as 0.5 doubling after transfer to light medium. Our results are compatible with a conservative mode of replication or with a semiconservative mode of replication carried out by a small portion of the total dsRNA population. In additional experiments the synthesis of dsRNA relative to the cell cycle was studied. This was done by determining the ratio of short-term to long-term radioactive label in size-separated cell fractions of a prelabeled exponential culture. The ratio of short-term to long-term label remained constant for all fractions, implying that dsRNA is synthesized throughout the cell cycle, increasing through the cell cycle at an exponential rate. |
| Databáze: | OpenAIRE |
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