Ehrlichia canis phylogenetic analysis of the Borgo (Corsica) strain
| Autor: | J.-C. Audonnet, Bernard Davoust, T. Normand, D. Parzy, P. Baudu |
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| Rok vydání: | 2009 |
| Předmět: |
Microbiology (medical)
DNA Bacterial Ehrlichia canis Biology Tick DNA Ribosomal Dogs Phylogenetics RNA Ribosomal 16S Sequence Homology Nucleic Acid Genotype Animals Cluster Analysis Point Mutation Dog Diseases Phylogeny Genetics Ehrlichiosis General Medicine Sequence Analysis DNA Ribosomal RNA biology.organism_classification 16S ribosomal RNA Virology Rhipicephalus Canis Infectious Diseases Blood France |
| Zdroj: | Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases. 15 |
| ISSN: | 1469-0691 |
| Popis: | Direction Re´gionale du Service de Sante´ des Arme´esde Toulon, Toulon Arme´es, FranceEhrlichia canis (E. canis), a Gram-negative obliga-tory intracellular bacterium with a tropism formonocytes and macrophages, is acknowledged asthe primary cause of canine monocytic ehrlichio-sis (CME). First described in 1935 in Algeria byDonatien and Lestoquard, the worldwide diffu-sion of this agent is related to the distribution ofthe brown dog tick, Rhipicephalus sanguineus.Particularly frequent in tropical and subtropicalregions, this tick is also widely present in theMediterranean Basin. Although CME was firstreported in France in the late 1970s, no molecularcharacterisation of E. canis strains originatingfrom a dog has ever been attempted. The purposeof the present study was to characterise the 16SrRNA genotype of E. canis strains naturallyinfecting French dogs and to compare this strainwith those from other regions. The isolation of thestrain E. canis Borgo from a dog with a history ofchronic CME from Borgo (Corsica) has allowedthe study of his pathogenesis and molecularcharacterisation.Blood samples were drawn from a dog withclinical and haematological signs of ehrlichiosisand inoculated into healthy dogs. Blood asepti-cally collected from the infected dogs was inoc-ulated into MDHSP cells for culture isolation.Genomic DNA was extracted from this cellculture and the 16S rRNA gene of E. canis wasamplified by reverse transcription PCR as previ-ously described and sequenced with an automaticDNA sequencer.Multiple sequence alignments with the refer-ence sequences downloaded from GenBank wereperformed with the |
| Databáze: | OpenAIRE |
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