Flow Cytometric Analysis of Lymphoid Enhancer-Binding Factor 1 in Diagnosis of Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma
Autor: | LoAnn Peterson, Juehua Gao, Kristy L. Wolniak, Yi Hua Chen, Charles L. Goolsby, Janet L. McLaughlin, Catalina Amador-Ortiz |
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Rok vydání: | 2015 |
Předmět: |
Adult
Male Pathology medicine.medical_specialty Lymphoid Enhancer-Binding Factor 1 Chronic lymphocytic leukemia Flow cytometry immune system diseases hemic and lymphatic diseases Biomarkers Tumor medicine Humans B-cell lymphoma Aged Aged 80 and over medicine.diagnostic_test business.industry General Medicine T lymphocyte Middle Aged Flow Cytometry medicine.disease Immunohistochemistry Leukemia Lymphocytic Chronic B-Cell Lymphoma Staining embryonic structures Female business Lymphoid enhancer-binding factor 1 |
Zdroj: | American Journal of Clinical Pathology. 143:214-222 |
ISSN: | 1943-7722 0002-9173 |
Popis: | Objectives: Nuclear overexpression of lymphoid enhancer-binding factor 1 (LEF1) assessed by immunohistochemistry has been shown to be highly associated with chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) among small B-cell lymphomas. The purpose of this study was to evaluate the utility of flow cytometric analysis of LEF1 in the diagnosis of CLL/SLL. Methods: Normal peripheral blood was used to validate the test. Flow cytometric analysis of LEF1 was performed in 64 patient samples qualitatively and quantitatively by comparing the staining intensity and the ratios of the median fluorescence intensities (MFIs) of LEF1 in B cells of interest to the internal reference cell populations. The results were correlated with the pathologic diagnosis. Results: Proper sample processing ensured sufficient separation of positive LEF1 staining in T cells from negative staining in normal B and natural killer (NK) cells. Qualitative analysis of patient samples showed that all 25 cases of CLL/SLL but none of the other small B-cell lymphomas were positive for LEF1. Using a B/NK MFI ratio of 1.5 and B/T MFI ratio of 0.45 separated CLL/SLL cases from non-CLL lymphomas. Conclusions: Flow cytometric analysis of LEF1 is sufficient to differentiate CLL/SLL from other small B-cell lymphomas and may serve as a useful tool in the diagnosis of CLL/SLL. |
Databáze: | OpenAIRE |
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